NDLI: Statistical optimization of cellulases production by Penicillium chrysogenum QML-2 under solid-state fermentation and primary application to chitosan hydrolysis

Content Provider	Springer Nature Link
Author	Zhang, Hui Sang, Qing
Copyright Year	2011
Abstract	Solid-state fermentation conditions for cellulases production by a newly isolated Penicillium chrysogenum QML-2 were investigated using statistical methods. At first, significant variables for cellulases production including (NH$_{4}$)$_{2}$SO$_{4}$, initial pH and inoculum size were screened by using Plackett-Burman Design. Then the optimal regions of the significant variables were investigated by using the method of steepest ascent. Finally, central composite design and response surface analysis were adopted to determine the optimal values of the significant variables and investigate the combined effects of each variable’s pair on cellulases production. The results showed that the optimal ranges of (NH$_{4}$)$_{2}$SO$_{4}$ concentration, initial pH and inoculum size for three types of cellulases activities were 1.97–2.15 g, pH 4.32–4.41 and 13.3–13.7% (v/w), respectively. Using the mixture of corn stover powder and wheat bran (CSP/WB, 1/1) as carbon source, the optimization resulted in 370.15, 101.76 and 321.56 U/g for maximal endoglucanase activity, filter paper activity and β-glucosidase activity, respectively. Compared with maximum values of cellulases activities (endoglucanase activity 85.21 U/g, filter paper activity 16.62 U/g and β-glucosidase activity 67.68 U/g) obtained under unoptimized conditions, the optimization resulted in 3.34, 5.12 and 3.75 folds improvement for endoglucanase activity, filter paper activity and β-glucosidase activity, respectively. For chitosan hydrolysis, the crude cellulases had the optimal temperature of 55°C, pH of 4.4 and exhibited Michaelis constant (K $_{m}$) value of 8.34 mg/ml and maximum velocity (V $_{max}$) of 2.21 μmol glucosamine/min by 1 ml of the crude cellulases.
Starting Page	1163
Ending Page	1174
Page Count	12
File Format	PDF
ISSN	09593993
Journal	World Journal of Microbiology and Biotechnology
Volume Number	28
Issue Number	3
e-ISSN	15730972
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-10-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cellulases Penicillium chrysogenum Solid-state fermentation Response surface methodology Optimization Chitosan hydrolysis Biotechnology Microbiology Biochemistry Applied Microbiology Environmental Engineering/Biotechnology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Statistical optimization of cellulases production by Penicilliumchrysogenum QML-2 under solid-state fermentation and primary application to chitosan hydrolysis

Statistical optimization of cellulases production by Aspergillus niger HQ-1 in solid-state fermentation and partial enzymatic characterization of cellulases on hydrolyzing chitosan

Application of response surface methodology in medium optimization for spore production of Coniothyrium minitans in solid-state fermentation

Thermoactive β-N-acetylhexosaminidase production by a soil isolate of Penicillium monoverticillium CFR 2 under solid state fermentation: parameter optimization and application for N-acetyl chitooligosaccharides preparation from chitin

Cellulase with high β-glucosidase activity by Penicillium oxalicum under solid state fermentation and its use in hydrolysis of cassava residue

Application of statistical tool for optimization of physical parameters for cellulase production under solid state fermentation

Production of a novel raw-starch-digesting glucoamylase by Penicillium sp. X-1 under solid state fermentation and its use in direct hydrolysis of raw starch

Optimization of fermentation conditions for production of xylanase by a newly isolated strain, Penicillium thiersii ZH-19

Amylase Production by Saccharomycopsis fibuligera A11 in Solid-State Fermentation for Hydrolysis of Cassava Starch

Statistical optimization of cellulases production by Penicillium chrysogenum QML-2 under solid-state fermentation and primary application to chitosan hydrolysis

Similar Documents

Statistical optimization of cellulases production by Penicilliumchrysogenum QML-2 under solid-state fermentation and primary application to chitosan hydrolysis

Statistical optimization of cellulases production by Aspergillus niger HQ-1 in solid-state fermentation and partial enzymatic characterization of cellulases on hydrolyzing chitosan

Application of response surface methodology in medium optimization for spore production of Coniothyrium minitans in solid-state fermentation

Thermoactive β-N-acetylhexosaminidase production by a soil isolate of Penicillium monoverticillium CFR 2 under solid state fermentation: parameter optimization and application for N-acetyl chitooligosaccharides preparation from chitin

Cellulase with high β-glucosidase activity by Penicillium oxalicum under solid state fermentation and its use in hydrolysis of cassava residue

Application of statistical tool for optimization of physical parameters for cellulase production under solid state fermentation

Production of a novel raw-starch-digesting glucoamylase by Penicillium sp. X-1 under solid state fermentation and its use in direct hydrolysis of raw starch

Optimization of fermentation conditions for production of xylanase by a newly isolated strain, Penicillium thiersii ZH-19

Amylase Production by Saccharomycopsis fibuligera A11 in Solid-State Fermentation for Hydrolysis of Cassava Starch

Statistical optimization of cellulases production by Penicillium chrysogenum QML-2 under solid-state fermentation and primary application to chitosan hydrolysis