NDLI: Horizontal gene transfer of stress resistance genes through plasmid transport

Content Provider	Springer Nature Link
Author	Shoeb, Erum Badar, Uzma Akhter, Jameela Shams, Hina Sultana, Maria Ansari, Maqsood A.
Copyright Year	2011
Abstract	The horizontal gene transfer of plasmid-determined stress tolerance was achieved under lab conditions. Bacterial isolates, Enterobacter cloacae (DGE50) and Escherichia coli (DGE57) were used throughout the study. Samples were collected from contaminated marine water and soil to isolate bacterial strains having tolerance against heavy metals and antimicrobial agents. We have demonstrated plasmid transfer, from Amp$^{+}$Cu$^{+}$Zn$^{−}$ strain (DGE50) to Amp$^{−}$Cu$^{−}$Zn$^{+}$ strain (DGE57), producing Amp$^{+}$Cu$^{+}$Zn$^{+}$ transconjugants (DGE$_{TC50→57}$) and Amp$^{+}$Cu$^{−}$Zn$^{+}$ transformants (DGE$_{TF50→57}$). DGE57 did not carry any plasmid, therefore, it can be speculated that zinc tolerance gene in DGE57 is located on chromosome. DGE50 was found to carry three plasmids, out of which two were transferred through conjugation into DGE57, and only one was transferred through transformation. Plasmid transferred through transformation was one out of the two transferred through conjugation. Through the results of transformation it was revealed that the genes of copper and ampicillin tolerance in DGE50 were located on separate plasmids, since only ampicillin tolerance genes were transferred through transformation as a result of one plasmid transfer. By showing transfer of plasmids under lab conditions and monitoring retention of respective phenotype via conjugation and transformation, it is very well demonstrated how multiple stress tolerant strains are generated in nature.
Starting Page	1021
Ending Page	1025
Page Count	5
File Format	PDF
ISSN	09593993
Journal	World Journal of Microbiology and Biotechnology
Volume Number	28
Issue Number	3
e-ISSN	15730972
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-09-29
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Antimicrobial Heavy metals Multiple stress Conjugation Transformation Biotechnology Microbiology Biochemistry Applied Microbiology Environmental Engineering/Biotechnology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Horizontal gene transfer of stress resistance genes through plasmid transport.

Horizontal transfer of non-conjugative plasmid in colony biofilm of Escherichia coli on food-based media

Multiple plasmid origin‐of‐transfer regions might aid the spread of antimicrobial resistance to human pathogens

Lateral gene transfer occurring in haloarchaea: an interpretative imitation study

Ecotoxic heavy metals transformation by bacteria and fungi in aquatic ecosystem

Editorial: Horizontal Gene Transfer Mediated Bacterial Antibiotic Resistance

Dissemination of Antimicrobial Resistance in Microbial Ecosystems through Horizontal Gene Transfer

Assessment of a plasmid conjugation procedure to monitor horizontal transfer of an extended-spectrum β-lactamase resistance gene under food chain scenarios.

Host-specific factors determine the persistence of IncP-1 plasmids

Horizontal gene transfer of stress resistance genes through plasmid transport

Similar Documents

Horizontal gene transfer of stress resistance genes through plasmid transport.

Horizontal transfer of non-conjugative plasmid in colony biofilm of Escherichia coli on food-based media

Multiple plasmid origin‐of‐transfer regions might aid the spread of antimicrobial resistance to human pathogens

Lateral gene transfer occurring in haloarchaea: an interpretative imitation study

Ecotoxic heavy metals transformation by bacteria and fungi in aquatic ecosystem

Editorial: Horizontal Gene Transfer Mediated Bacterial Antibiotic Resistance

Dissemination of Antimicrobial Resistance in Microbial Ecosystems through Horizontal Gene Transfer

Assessment of a plasmid conjugation procedure to monitor horizontal transfer of an extended-spectrum β-lactamase resistance gene under food chain scenarios.

Host-specific factors determine the persistence of IncP-1 plasmids

Horizontal gene transfer of stress resistance genes through plasmid transport