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| Content Provider | Springer Nature Link |
|---|---|
| Author | Fiocco, D. Crisetti, E. Capozzi, V. Spa, G. |
| Copyright Year | 2007 |
| Abstract | The dissection of the stress tolerance in lactic acid bacteria (LAB) may improve our knowledge on the adaptive physiology of LAB and may facilitate the selection of species suitable for industrial application. In this work we report the validation of the ldhD gene as internal control for reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis in a model LAB such as Lactobacillus plantarum. The expression of the ldhD gene was analysed in lag phase, early, mid and late exponential phase and early and late stationary phase. Moreover, different stress conditions were analysed: heat shock (40°C), cold shock (10°C) and ethanolic shock (10% v/v). The quantification of transcript levels of a small heat shock gene (hsp 18.55) after heat, cold and ethanol shock allowed us to validate this method. RT-qPCR appeared as a powerful tool to study L. plantarum response in stress conditions and the internal control used in this study may be extended to other species of LAB. |
| Starting Page | 899 |
| Ending Page | 902 |
| Page Count | 4 |
| File Format | |
| ISSN | 09593993 |
| Journal | World Journal of Microbiology and Biotechnology |
| Volume Number | 24 |
| Issue Number | 6 |
| e-ISSN | 15730972 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2007-09-20 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | RT-qPCR Lactobacillus plantarum Small heat shock genes ldhD Microbiology Environmental Engineering/Biotechnology Biochemistry Biotechnology Applied Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Medicine Applied Microbiology and Biotechnology Biotechnology |
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