NDLI: Effect of additives on the esterification activity of immobilized Candida antarctica lipase

Content Provider	Springer Nature Link
Author	Rodrigues, Dasciana S. Cavalcante, George P. Silva, Giovanilton F. Ferreira, Andrea L. O. Gonçalves, Luciana R. B.
Copyright Year	2007
Abstract	In this work, the stabilizing effect of bovine serum albumin (BSA), peptone (PEP), and polyethylene glycol (PEG) during immobilization of Candida antarctica lipase on activated carbon was investigated. The influence of enzyme concentration and type of additive, added during the immobilization procedure, was studied using a 2$^{2}$ factorial central composite design. The goal was to maximize the synthetic activity of butyl butyrate, using butyric acid and butanol as substrate in n-heptane. An increase of 31–58% in the esterification activity was obtained when enzyme concentration on the supernatant was enhanced from 86.50 U m L$^{−1}$ to 226.80 U mL$^{−1}$. An enhancement in esterification activity of 38–68.95% was observed, depending on the initial enzyme concentration, when PEP was used instead of BSA. No significant increase in the esterification activity was observed when PEP was replaced by PEG. However, thermal stability tests at 50 °C showed that PEG had a higher stabilizing effect.
Starting Page	833
Ending Page	839
Page Count	7
File Format	PDF
ISSN	09593993
Journal	World Journal of Microbiology and Biotechnology
Volume Number	24
Issue Number	6
e-ISSN	15730972
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-09-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Candida antarctica lipase B Enzyme immobilization Activated carbon Adsorption and additives Microbiology Environmental Engineering/Biotechnology Biochemistry Biotechnology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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