NDLI: Mamestra configurata nucleopolyhedrovirus-A transcriptome from infected host midgut

Content Provider	Springer Nature Link
Author	Donly, B. Cameron Theilmann, David A. Hegedus, Dwayne D. Baldwin, Douglas Erlandson, Martin A.
Copyright Year	2013
Abstract	Infection of an insect by a baculovirus occurs in two distinct phases, an initial infection of host midgut by occlusion-derived virions (ODVs) and subsequent systemic infection of other tissues by budded virions (BV). A vast majority of investigations of the infection process have been restricted to cell culture studies using BV that emulate the systemic phase of infection. This is one of the first studies to investigate baculovirus gene expression in ODV infected midgut cells. We have focused on the critical first phase of in vivo infection by Mamestra configurata nucleopolyhedrovirus-A in M. configurata larvae, using qPCR and RNAseq mass sequencing to measure virus gene expression in midgut cells. The earliest genes detected by each method had significant overlap, including known early genes as well as genes unique to MacoNPV-A and genes of unknown function. The RNAseq data also revealed a large range of expression levels across all ORFs, which could not be measured using qPCR. This dataset provides a first whole genome transcriptomic analysis of viral genes required for virus infection in vivo and will provide the basis for functionally analyzing specific genes that may be critical elements in baculovirus midgut infectivity and host range.
Starting Page	174
Ending Page	183
Page Count	10
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	48
Issue Number	1
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2013-10-15
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mamestra configurata Baculovirus Transcriptome RNAseq qPCR Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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