NDLI: Functional analysis of Spodoptera litura nucleopolyhedrovirus p49 gene during Autographa californica nucleopolyhedrovirus infection of SpLi-221 cells

Content Provider	Springer Nature Link
Author	Lin, Tiehao Yu, Mei Wu, Wenbi Yu, Qian Weng, Qingbei Yang, Kai Yuan, Meijin Pang, Yi
Copyright Year	2010
Abstract	The Spodoptera litura nucleopolyhedrovirus (SpltNPV) antiapoptotic gene Splt-p49 is able to rescue replication of a p35-null Autographa californica nucleopolyhedrovirus (AcMNPV) in AcMNPV-permissive Sf9 cells. In this study, an AcMNPV p35 knockout bacmid was generated through ET homologous recombination in Escherichia coli and designated as vAc$^{P35-KO}$. The Splt-p49 gene was transposed into the polyhedrin locus of vAc$^{P35-KO}$ to investigate if Splt-p49 has any antiapoptotic activity in the context of AcMNPV infection of AcMNPV-nonpermissive SpLi-221 cells. Our results demonstrated that Splt-p49 could not inhibit the apoptosis induced by AcMNPV infection of SpLi-221 cells when it was under the control of its native promoter. Western blot analysis showed that Splt-P49 was poorly expressed. When the expression of Splt-P49 was under the control of Drosophila hsp70 promoter, the expression of Splt-P49 was advanced, and a higher level of Splt-P49 was detected. As a result, the apoptosis of SpLi-221 cells was inhibited; however, budded-virus production did not improve in comparison with that in AcMNPV-infected SpLi-221 cells. These data indicated that there are other barrier(s) to AcMNPV replication in the nonpermissive SpLi-221 cells besides apoptosis.
Starting Page	441
Ending Page	449
Page Count	9
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	41
Issue Number	3
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2010-08-15
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Apoptosis Antiapoptotic gene Baculovirus Host range Plant Sciences Virology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Identification of the Apoptosis Inhibitor Gene p49 of Spodoptera litura Multicapsid Nucleopolyhedrovirus

The Function of Envelope Protein P74 from Autographa californica Multiple Nucleopolyhedrovirus in Primary Infection to Host

Characterization of Antheraea pernyi nucleopolyhedrovirus p11 gene, a homologue of Autographa californica nucleopolyhedrovirus orf108

Identification of Insertion and Deletion Genes in Autographa californica Nucleopolyhedrovirus Variants Isolated from Galleria mellonella, Spodoptera exigua, Spodoptera litura and Xestia c-Nigrum

Comparative proteomics analysis of Spodoptera frugiperda cells during Autographa californica multiple nucleopolyhedrovirus infection

Encyclopedia of Autographa californica nucleopolyhedrovirus genes

Identification and characterization of odv-e25 of Spodoptera litura multicapsid nucleopolyhedrovirus

Infection of wild-type Autographa californica multicapsid nucleopolyhedrovirus induces in vivo apoptosis of Spodoptera litura larvae (2002)

Infection of wild-type Autographa californica multicapsid nucleopolyhedrovirus induces in vivo apoptosis of Spodoptera litura larvae.

Functional analysis of Spodoptera litura nucleopolyhedrovirus p49 gene during Autographa californica nucleopolyhedrovirus infection of SpLi-221 cells

Similar Documents

Identification of the Apoptosis Inhibitor Gene p49 of Spodoptera litura Multicapsid Nucleopolyhedrovirus

The Function of Envelope Protein P74 from Autographa californica Multiple Nucleopolyhedrovirus in Primary Infection to Host

Characterization of Antheraea pernyi nucleopolyhedrovirus p11 gene, a homologue of Autographa californica nucleopolyhedrovirus orf108

Identification of Insertion and Deletion Genes in Autographa californica Nucleopolyhedrovirus Variants Isolated from Galleria mellonella, Spodoptera exigua, Spodoptera litura and Xestia c-Nigrum

Comparative proteomics analysis of Spodoptera frugiperda cells during Autographa californica multiple nucleopolyhedrovirus infection

Encyclopedia of Autographa californica nucleopolyhedrovirus genes

Identification and characterization of odv-e25 of Spodoptera litura multicapsid nucleopolyhedrovirus

Infection of wild-type Autographa californica multicapsid nucleopolyhedrovirus induces in vivo apoptosis of Spodoptera litura larvae (2002)

Infection of wild-type Autographa californica multicapsid nucleopolyhedrovirus induces in vivo apoptosis of Spodoptera litura larvae.

Functional analysis of Spodoptera litura nucleopolyhedrovirus p49 gene during Autographa californica nucleopolyhedrovirus infection of SpLi-221 cells