NDLI: Identification of a conserved B-cell epitope in the equine arteritis virus (EAV) N protein using the pepscan technique

Content Provider	Springer Nature Link
Author	Zhao, Shihua Qi, Ting Guo, Wei Lu, Gang Xiang, Wenhua
Copyright Year	2013
Abstract	The nucleocapsid (N) gene of equine arteritis virus (EAV) is highly conserved between isolates, and the N protein is an important antigen that induces immunity when horses are infected with EAV. This study describes the identification of a linear B-cell epitope on the N protein using the pepscan technique with a monoclonal antibody (mAb) 2B1 directed against the N protein. The N protein was divided into 11 overlapping peptides, each containing 16 amino acids associated with six overlapping amino acids. The fragments were expressed as MBP fusion proteins that were then used to probe the 2B1 mAb. The minimal epitope sequence was confirmed step-by-step using single amino acid residue deletion. One completely conserved linear epitope ($^{38}$KPPAQP$^{43}$) was identified that matched with EAV-positive serum in Western blots, thereby revealing the importance of these six amino acids of the epitope for antibody-epitope binding activity. This finding not only contributes to our understanding of the antigenic structure of the N protein of EAV but also has potential for the development of diagnostic techniques.
Starting Page	292
Ending Page	297
Page Count	6
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	47
Issue Number	2
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2013-06-28
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Epitope N Protein Pepscan technique EAV Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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