NDLI: The transcription analysis of duck enteritis virus UL49.5 gene using real-time quantitative reverse transcription PCR

Content Provider	Springer Nature Link
Author	Lin, Meng Jia, Renyong Wang, Mingshu Gao, Xinghong Zhu, Dekang Chen, Shun Yin, Zhongqiong Wang, Yin Chen, Xiaoyue Cheng, Anchun
Copyright Year	2013
Abstract	Duck enteritis virus (DEV) UL49.5 encoding glycoprotein N was a conserved gene. The transcription dynamic process of UL49.5 homologous genes in herpesviruses was reported. However, the transcription dynamic process of DEV UL49.5 gene has not yet been established. In this study, a real-time quantitative reverse transcription PCR (real-time qRT-PCR) assay was established to test the transcription dynamic process of DEV UL49.5 gene, and the recombinant plasmid pUCm-T/UL49.5 was constructed as the standard DNA. The samples prepared from DEV-infected (at different time points) and uninfected cell were detected and calculated. The results demonstrated that the real-time qRT-PCR assay was successfully established. The transcription product of DEV UL49.5 gene was first detected at 0.5 h post infection (p.i.), increased at 8 h p.i. and reached a peak at 60 h p.i. Our results illustrated that DEV UL49.5 gene could be regarded as a late gene. The transcription dynamic process of DEV UL49.5 gene may provide a significant clue for further studies of DEV UL49.5 gene.
Starting Page	298
Ending Page	304
Page Count	7
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	47
Issue Number	2
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2013-07-09
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Duck enteritis virus UL49.5 gene Molecular cloning Real-time qRT-PCR Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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