NDLI: Molecular analysis of grass carp reovirus HZ08 genome segments 1–3 and 5

Content Provider	Springer Nature Link
Author	Zhang, Chao Wang, Qing Shi, Cunbin Zeng, Weiwei Liu, Yongkui Wu, Shuqin
Copyright Year	2010
Abstract	A new grass carp reovirus, assigned HZ08, was isolated from a diseased grass carp case during routine examination in Huzhou City, Zhejiang Province, China. The complete nucleotide sequences of genomic segments S1–S3 and S5–S6 were obtained and comprised 3927, 3870, 3753, 2229, and 2030 bp, respectively. Each segment contained a single open reading frame which encoded putative proteins of 143.6, 143.1, 135.9, 80.5, and 68.4 kDa, respectively. Conserved motifs 5′ (GUAAUUU…UUCAUC) 3′ were found at the ends of each segment. At the amino acid level, HZ08 S1–S3 and S5–S6 showed similarity to the corresponding segments of Aquareovirus. Further phylogenetic analysis using the amino acid sequences of the RNA-dependent RNA polymerases protein encoded by S2 revealed that HZ08 formed a cluster close to the aquareovirus, but was far from the other isolates, which indicated that HZ08 is likely to be a new member of Aquareovirus.
Starting Page	102
Ending Page	104
Page Count	3
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	41
Issue Number	1
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2010-05-09
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Grass carp reovirus Aquareovirus Sequencing Phylogenetic analysis Plant Sciences Virology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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