NDLI: Molecular Cloning of Coat Protein Gene of an Indian Cotton Leaf Curl Virus (CLCuV-HS2) Isolate and its Phylogenetic Relationship with others Members of Geminiviridae

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Molecular Cloning of Coat Protein Gene of an Indian Cotton Leaf Curl Virus (CLCuV-HS2) Isolate and its Phylogenetic Relationship with others Members of Geminiviridae

Content Provider	Springer Nature Link
Author	Sharma, Pradeep Rishi, Narayan Malathi, V. G.
Copyright Year	2005
Abstract	Cotton leaf curl geminivirus is a whitefly (Bemisia tabaci Genn.) transmitted Begomovirus (Family Geminiviridae) causing a serious disease of cotton in northern India. The very typical symptoms of present isolate (CLCuV-HS2) showed thickened veins, dark green discoloration of the leaves with upward curling and leaf like structure known as enation (one in number), which develops into cup-shaped on the reverse side of leaves. The polymerase chain reaction (PCR) based technique can detect the viral DNA in samples stored upto 3 days after the collection and have wide application for the field diagnosis. The complete nucleotide sequence of the Indian isolate of cotton leaf curl geminivirus (CLCuV-HS2) coat protein (CP) gene component was determined using CP specific primers through PCR amplification from field infected cotton plants growing in Haryana, India. Comparison of the amino acid sequence of the putative CP with some other mono and bipartite geminiviruses revealed a maximum of 97.3% identity with Pakistan cotton leaf curl virus (CLCuV-62). A nuclear localization signal located close to the N-terminal of CP gene was determined.
Starting Page	85
Ending Page	91
Page Count	7
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	30
Issue Number	1
e-ISSN	1572994X
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bemisia tabaci cloning cotton leaf curl virus polymerase chain reaction whitefly Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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