NDLI: Variable Regions 1 and 2 (VR1 and VR2) in JSRV gag Are Not Responsible for the Endogenous JSRV Particle Release Defect

Please wait, while we are loading the content...

Viral Sequence Insertions and a Novel Cellular Insertion in the NS2 Gene of Cytopathic Isolates of Bovine Viral Diarrhea Virus as Potential Cytopathogenicity Markers

Variable Regions 1 and 2 (VR1 and VR2) in JSRV gag Are Not Responsible for the Endogenous JSRV Particle Release Defect

Cloning and Sequence Analysis of the M gene of Porcine Epidemic Diarrhea Virus LJB/03

Identification of a Naturally Occurring Recombinant Isolate of Sugarcane Mosaic Virus Causing Maize Dwarf Mosaic Disease

Molecular Cloning of Coat Protein Gene of an Indian Cotton Leaf Curl Virus (CLCuV-HS2) Isolate and its Phylogenetic Relationship with others Members of Geminiviridae

Isolation of Virus from a SARS Patient and Genome-wide Analysis of Genetic Mutations Related to Pathogenesis and Epidemiology from 47 SARS-CoV Isolates

Cumulative Mutations in the Genome of Echovirus 6During Establishment of a Chronic Infection in Precursors of Glial Cells

Completion of the Sequence of a Cetacean Morbillivirus and Comparative Analysis of the Complete Genome Sequences of Four Morbilliviruses

Binding of Cellular Proteins to the Leader RNA of Equine Arteritis Virus

A Point of View: HIV-1/AIDS is an Allergy but CpG ODN Treatments may Inhibit Virus Replication and Reactivate the Adaptive Immunity – Hypothesis and Implications

Virus Genes : Volume 29

Virus Genes : Volume 28

Virus Genes : Volume 27

Virus Genes : Volume 26

Virus Genes : Volume 25

Virus Genes : Volume 24

Virus Genes : Volume 23

Virus Genes : Volume 22

Virus Genes : Volume 21

Virus Genes : Volume 20

Virus Genes : Volume 19

Virus Genes : Volume 18

Virus Genes : Volume 17

Virus Genes : Volume 16

Virus Genes : Volume 15

Virus Genes : Volume 14

Variable Regions 1 and 2 (VR1 and VR2) in JSRV gag Are Not Responsible for the Endogenous JSRV Particle Release Defect

Content Provider	Springer Nature Link
Author	Hallwirth, Claus Maeda, Naoyoshi York, Denis Fan, Hung
Copyright Year	2005
Abstract	Jaagsiekte sheep retrovirus (JSRV) is a betaretrovirus causing ovine pulmonary adenocarcinoma, a transmissible lung tumor of sheep. A very closely related endogenous retrovirus (enJSRV) occurs as 15 to 20 copies in the genome of all sheep, and is not known to be linked to pathogenesis. We previously localized a particle release defect of the full-length endogenous-derived expression construct pCMV2enJS56A1 to the amino-terminal region of gag that incorporates the two variable regions VR1 and VR2, which harbor the main sequence differences between endogenous and exogenous JSRV in this part of gag. Here, we tested the hypothesis that either or both of these variable regions are responsible for the observed particle release defect in enJS56A1. We found that the PPPPPPPS motif of the exogenous VR1 is neither necessary nor sufficient for particle release. Furthermore, the precise substitution of VR1 and VR2 in the exogenous JSRV expression plasmid pCMV2JS $_{21}$, using their enJS56A1-derived counterparts, did not abrogate the ability of the resulting constructs to release particles. The particle release defect of enJS56A1 is therefore not determined exclusively by either VR1 or VR2. These results point to a small number of amino acids lying outside of VR1 and VR2 that may be responsible for the particle defect of enJS56A1 Gag.
Starting Page	59
Ending Page	68
Page Count	10
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	30
Issue Number	1
e-ISSN	1572994X
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	enJSRV Jaagsiekte sheep retrovirus ovine pulmonary adenocarcinoma particle release sheep substitution Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Molecular Diagnosis of Jaagsiekte Sheep Retrovirus (JSRV) in Sheep affected by Ovine Pulmonary Adenocarcinoma

Cloning and sequence analysis of genome from the Inner Mongolia strain of the endogenous betaretroviruses (enJSRV)

PATHOLOGICAL STUDIES AND RT-PCR DETECTION OF JAAGSIEKTE SHEEP RETROVIRUS (JSRV) IN SHEEP IN NATURAL CASES OF OVINE PULMONARY ADENOCARCINOMA

Transcriptional Response of Ovine Lung to Infection with Jaagsiekte Sheep Retrovirus.

Jaagsiekte Sheep Retrovirus Biology and Oncogenesis

PATHOLOGICAL STUDIES AND RT-PCR DETECTION OF JAAGSIEKTE SHEEP RETROVIRUS (JSRV) IN SHEEP IN NATURAL CASES OF OVINE PULMONARY ADENOCARCINOMA

Molecular Diagnosis of Jaagsiekte Sheep Retrovirus (JSRV) in Sheep affected by Ovine Pulmonary Adenocarcinoma

First confirmation by PCR of Jaagsiekte sheep retrovirus in Ireland and prevalence of ovine pulmonary adenocarcinoma in adult sheep at slaughter

Jaagsiekte Sheep Retrovirus (JSRV): from virus to lung cancer in sheep

Variable Regions 1 and 2 (VR1 and VR2) in JSRV gag Are Not Responsible for the Endogenous JSRV Particle Release Defect

Similar Documents

Molecular Diagnosis of Jaagsiekte Sheep Retrovirus (JSRV) in Sheep affected by Ovine Pulmonary Adenocarcinoma

Cloning and sequence analysis of genome from the Inner Mongolia strain of the endogenous betaretroviruses (enJSRV)

PATHOLOGICAL STUDIES AND RT-PCR DETECTION OF JAAGSIEKTE SHEEP RETROVIRUS (JSRV) IN SHEEP IN NATURAL CASES OF OVINE PULMONARY ADENOCARCINOMA

Transcriptional Response of Ovine Lung to Infection with Jaagsiekte Sheep Retrovirus.

Jaagsiekte Sheep Retrovirus Biology and Oncogenesis

PATHOLOGICAL STUDIES AND RT-PCR DETECTION OF JAAGSIEKTE SHEEP RETROVIRUS (JSRV) IN SHEEP IN NATURAL CASES OF OVINE PULMONARY ADENOCARCINOMA

Molecular Diagnosis of Jaagsiekte Sheep Retrovirus (JSRV) in Sheep affected by Ovine Pulmonary Adenocarcinoma

First confirmation by PCR of Jaagsiekte sheep retrovirus in Ireland and prevalence of ovine pulmonary adenocarcinoma in adult sheep at slaughter

Jaagsiekte Sheep Retrovirus (JSRV): from virus to lung cancer in sheep

Variable Regions 1 and 2 (VR1 and VR2) in JSRV gag Are Not Responsible for the Endogenous JSRV Particle Release Defect