NDLI: Molecular cloning, sequence analysis and expression of genome segment 7 (S7) of Antheraea mylitta cypovirus (AmCPV) that encodes a viral structural protein

Please wait, while we are loading the content...

Transmembrane protein polymorphisms and resistance to T-20 (Enfuvirtide, Fuzeon$^{®}$) in HIV-1 infected therapy-naive seroconverters and AIDS patients under HAART-T-20 therapy

Computational analysis and identification of amino acid sites in dengue E proteins relevant to development of diagnostics and vaccines

Hydrophilicity dependent budding and secretion of chimeric HIV Gag-V3 virus-like particles

High-resolution analysis of CpG methylation and in vivo protein–DNA interactions at the alternative Epstein-Barr virus latency promoters Qp and Cp in the nasopharyngeal carcinoma cell line C666-1

Role of a consensus AP-2 regulatory sequence within the Epstein-Barr Virus LMP1 promoter in EBNA2 mediated transactivation

Induction of chromosomally integrated HIV-1 LTR requires RBF-2 (USF/TFII-I) and RAS/MAPK signaling

Minimal protein domain requirements for the intracellular localization and self-aggregation of Epstein-Barr Virus Latent Membrane Protein 2

Sequence and phylogenetic analysis of the S1 genome segment of turkey-origin reoviruses

Complete genomes and phylogenetic positions of bovine papillomavirus type 8 and a variant type from a European bison

Evolution of feline immunodeficiency virus Gag proteins

Heterologous expression of Bluetongue VP2 viral protein fragment in Pichia pastoris

Molecular relationships between type Asia 1 new strain from China and type O Panasia strains of foot-and-mouth-disease virus

Detection of SRV/D shedding in body fluids of cynomolgus macaques and comparison of partial gp70 sequences in SRV/D-T isolates

The pattern of viral persistence in monkeys intra-tracheally infected with Simian varicella virus

Genomic sequence of an antigenic variant Newcastle disease virus isolated in Korea

Comparison of LTR enhancer elements in sheep betaretroviruses: insights into the basis for tissue-specific expression

Genetic typing of equine arteritis virus isolates from Argentina

Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea

Different genotypes of nephropathogenic infectious bronchitis viruses co-circulating in chicken population in China

Prevalence and molecular characterization of meq in feather follicular epithelial cells of Korean broiler chickens

Immunization of DNA vaccine encoding C3d-VP1 fusion enhanced protective immune response against foot-and-mouth disease virus

A point mutation changes the serotype of a Potato virus Y isolate; genomic determination of the serotype of PVY strains

Molecular characterization and phylogenetic relationships of two new bipartite begomovirus infecting malvaceous plants in Yucatan, Mexico

Molecular characterization of sweet potato leaf curl virus isolate from China (SPLCV-CN) and its phylogenetic relationship with other members of the Geminiviridae

Molecular analysis of six segments of Tobacco leaf enation virus, a novel phytoreovirus from tobacco

The Ryegrass mottle virus genome codes for a sobemovirus 3C-like serine protease and RNA-dependent RNA polymerase translated via −1 ribosomal frameshifting

Transmission of a dsRNA in bell pepper and evidence that it consists of the genome of an endornavirus

Molecular evidence and sequence analysis of a natural reassortant between Cucumber mosaic virus subgroup IA and II strains

Analysis of the molecular and biological variability of Zucchini yellow mosaic virus isolates from Slovakia and Czech Republic

Complete genomic sequence of Dracaena mottle virus, a distinct badnavirus

Molecular characterization of a severe isolate of papaya ringspot virus in Mexico and its relationship with other isolates

Molecular cloning, sequence analysis and expression of genome segment 7 (S7) of Antheraea mylitta cypovirus (AmCPV) that encodes a viral structural protein

Molecular characterization and genetic organization of the inhibitor of apoptosis gene (iap-5) region of the Pieris rapae granulovirus

Transcriptional profile of red seabream iridovirus in a fish model as revealed by viral DNA microarrays

Characterisation and partial sequence analysis of two novel cypoviruses isolated from the winter moth Operophtera brumata (Lepidoptera: Geometridae)

Virus Genes : Volume 35, Issue 1, August 2007

Virus Genes : Volume 34

Virus Genes : Volume 33

Virus Genes : Volume 32

Virus Genes : Volume 31

Virus Genes : Volume 30

Virus Genes : Volume 29

Virus Genes : Volume 28

Virus Genes : Volume 27

Virus Genes : Volume 26

Virus Genes : Volume 25

Virus Genes : Volume 24

Virus Genes : Volume 23

Virus Genes : Volume 22

Virus Genes : Volume 21

Virus Genes : Volume 20

Virus Genes : Volume 19

Virus Genes : Volume 18

Virus Genes : Volume 17

Virus Genes : Volume 16

Virus Genes : Volume 15

Virus Genes : Volume 14

Molecular cloning, sequence analysis and expression of genome segment 7 (S7) of Antheraea mylitta cypovirus (AmCPV) that encodes a viral structural protein

Content Provider	Springer Nature Link
Author	Chavali, Venkata Ramana Murthy Ghosh, Ananta K.
Copyright Year	2007
Abstract	The Genome segment 7 (S7) of the 11 double stranded RNA genomes from Antheraea mylitta cypovirus (AmCPV) was converted to cDNA, cloned and sequenced. The nucleotide sequence showed that segment 7 consisted of 1789 nucleotides with an ORF of 530 amino acids and could encode a protein of approximately 61 kDa, termed P61. The 5′ terminal sequence, AGTAAT and the 3′ terminal sequence, AGAGC of the plus strand was found to be the same as genome segment 10 of AmCPV encoding polyhedrin. No sequence similarity was found by searching nucleic acid and protein sequence databases using BLAST. The secondary structure prediction showed the presence of 17 α-helices, 18 extended β-sheets along the entire length of P61. The ORF of segment 7 was expressed in E. coli as His-tagged fusion protein, purified through Ni-NTA chromatography, and polyclonal antibody was raised in rabbit indicating that P61 is immunogenic. Immunoblot analysis using this antibody on viral infected cells as well as purified polyhedra showed that P61 is a viral structural protein. Motif scan search showed some similarity of P61 with Inosine monophosphate dehydrogenase (IMPDH) cystathionine-β-synthase (CBS) domain at the C-terminus and it was hypothesized that by binding to single stranded viral RNA through its CBS domain P61 may help in virus replication or transcription.
Starting Page	433
Ending Page	441
Page Count	9
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	35
Issue Number	2
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2007-01-26
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cypovirus Antheraea mylitta P61 Cloning Sequencing Recombinant protein expression CBS domain Plant Sciences Virology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Genome segment 5 of Antheraea mylitta cytoplasmic polyhedrosis virus encodes a bona fide guanylyltransferase

Molecular Analysis of Antheraea Mylitta Cytoplasmic Polyhedrosis Virus Genome Segments 4 and 5

Genome segment 4 of Antheraea mylitta cytoplasmic polyhedrosis virus encodes RNA triphosphatase and methyltransferases.

Molecular Cloning, DNA Sequence Analysis, and Expression of cDNA Sequence of RNA Genomic Segment 6 (S6) that Encodes a Viral Outer Capsid Protein of Threadfin Aquareovirus (TFV)

Genome segment 4 of Antheraea mylitta cytoplasmic polyhedrosis virus encodes RNA triphosphatase and methyltransferases.

Mori cypovirus 1, encoding the viral structural proteins v4 and.

Screening of Medicinal Plants Against the Infection of Antheraea mylitta Cytoplasdmic Polyhedrosis Virus (AmCPV) in Tropical Tasar Silkworm, Antheraea mylitta Drury Gajendra Pal Singh (2010)

Nucleotide sequence of genome segment 5 from Bombyx mori cypovirus 1

Reconstitution of the RNA-dependent RNA polymerase activity of Antheraea mylitta cypovirus in vitro using separately expressed different functional domains of the enzyme.

Molecular cloning, sequence analysis and expression of genome segment 7 (S7) of Antheraea mylitta cypovirus (AmCPV) that encodes a viral structural protein

Similar Documents

Genome segment 5 of Antheraea mylitta cytoplasmic polyhedrosis virus encodes a bona fide guanylyltransferase

Molecular Analysis of Antheraea Mylitta Cytoplasmic Polyhedrosis Virus Genome Segments 4 and 5

Genome segment 4 of Antheraea mylitta cytoplasmic polyhedrosis virus encodes RNA triphosphatase and methyltransferases.

Molecular Cloning, DNA Sequence Analysis, and Expression of cDNA Sequence of RNA Genomic Segment 6 (S6) that Encodes a Viral Outer Capsid Protein of Threadfin Aquareovirus (TFV)

Genome segment 4 of Antheraea mylitta cytoplasmic polyhedrosis virus encodes RNA triphosphatase and methyltransferases.

Mori cypovirus 1, encoding the viral structural proteins v4 and.

Screening of Medicinal Plants Against the Infection of Antheraea mylitta Cytoplasdmic Polyhedrosis Virus (AmCPV) in Tropical Tasar Silkworm, Antheraea mylitta Drury Gajendra Pal Singh (2010)

Nucleotide sequence of genome segment 5 from Bombyx mori cypovirus 1

Reconstitution of the RNA-dependent RNA polymerase activity of Antheraea mylitta cypovirus in vitro using separately expressed different functional domains of the enzyme.

Molecular cloning, sequence analysis and expression of genome segment 7 (S7) of Antheraea mylitta cypovirus (AmCPV) that encodes a viral structural protein