NDLI: Genetic analysis of Newcastle disease virus from Punjab, Pakistan

Content Provider	Springer Nature Link
Author	Shabbir, Muhammad Zubair Abbas, Muhammad Yaqub, Tahir Mukhtar, Nadia Subhani, Atta Habib, Hasham Sohail, Muhammad Umar Munir, Muhammad
Copyright Year	2012
Abstract	The strains of Newcastle disease virus (NDV) were isolated from five suspected outbreaks of ND in broiler (n = 3) and layers (n = 2) poultry farms. The egg-isolated viruses were subjected to biological and genetic characterization. Based on the biological characterization, isolates showed haemagglutination titer ≥log 2$^{7}$, mean death time <55 h, intracerebral pathogenecity index ≤1.8, and egg lethal dose 50 from 10$^{−7.15}$ to 10$^{−9.31}$/1 ml. Genetic characterization of the fusion (F) gene revealed that the isolates clustered with NDV strains of genotype VII (VIIf) within class II, which remained predominant genotype in the domestic poultry of Asia. The deduced amino acid sequence of the isolates confirmed virulent motif $^{112}$RRQKRF$^{117}$ at the F protein cleavage site. A bioinformatics and pairwise comparison approach was applied to estimate the synonymous and non-synonymous substitution rates (dN/dS) and selective evolutionary pressure for the F protein. The dN/dS calculated for genotype VII indicate purifying selection, which resulted in a low evolution rate in F gene. The F protein shows a strong negative pressure throughout the length of the protein and no recombination event was determined. Collectively, the results suggest that very similar virulent strains of NDV are involved during current wave of disease outbreak throughout the country. From these results, in conjunction with our recent reports of NDV from Pakistan, it is possible to conclude that emergence of novel group may require revisiting the diagnostics and vaccine control strategies.
Starting Page	309
Ending Page	315
Page Count	7
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	46
Issue Number	2
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2012-12-11
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Newcastle disease Fusion protein Phylogenetic analysis Evolutionary analysis Pakistan Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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