NDLI: The Conserved, 5′ Termini of RNAs 1 and 2 of Tomato aspermy virus are Dispensable for Infection but Affect Virulence

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The Conserved, 5′ Termini of RNAs 1 and 2 of Tomato aspermy virus are Dispensable for Infection but Affect Virulence

Content Provider	Springer Nature Link
Author	Shi, Bu Jun Palukaitis, Peter Symons, Robert H.
Copyright Year	2005
Abstract	The 5′ terminus of each of the three genomic RNAs (RNAs 1, 2 and 3) of Tomato aspermy virus (TAV) begins with the sequence 5′-GUUU, which is also shared by a number of other viruses. Mutagenic analyses showed that the 5′-GUUU sequence of RNAs 1 and 2 of TAV was dispensable for viral infection and did not prevent symptom induction. On the other hand, substitution of U at position 5 for G in RNA 1, but not RNA 2, induced veinal necrosis symptoms in Nicotiana glutinosa. The mutants constructed included insertion of UUU into the 5′-GUUU sequence of TAV RNAs 1 and/or 2. All RNA 2 mutants induced more severe symptoms than viral RNAs containing either mutated RNA 1 or most combinations of mutated RNAs 1 and 2. Some combinations of mutated RNAs 1 and 2 also induced veinal necrosis in N. glutinosa. Virulence was unrelated to the levels of viral RNA accumulation. Sequence analysis of progeny viral RNAs showed that only the mutant viral RNAs with a G to U substitution in RNA 1 and the deletion of the 5′-GUUU in both RNAs 1 and 2 were able to maintain the same sequence as the inoculum. The other mutants either reverted to the wildtype sequence or underwent further deletion or insertion. None of the constructed mutants were able to compete for accumulation with the wildtype virus after co-inoculation to the plant species tested.
Starting Page	181
Ending Page	191
Page Count	11
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	30
Issue Number	2
e-ISSN	1572994X
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	mutations non-translated region replication fidelity tomato aspermy virus Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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