NDLI: Molecular characterization of Newcastle disease viruses isolated from rural chicken in northwest Ethiopia reveals the circulation of three distinct genotypes in the country

Content Provider	Springer Nature Link
Author	Fentie, Tsegaw Heidari, Alireza Aiello, Roberta Kassa, Tesfu Capua, Ilaria Cattoli, Giovanni Sahle, Mesfin
Copyright Year	2013
Abstract	Newcastle disease (ND) is a highly contagious disease that affects many species of birds and causes significant economic losses to the poultry industry worldwide. Fifteen Newcastle disease virus (NDV) isolates obtained from rural chickens in northwest Ethiopia in 2011 and 2012 were characterized genotypically. The main functional region of the F gene was amplified and sequenced (260 nucleotides). Among the Ethiopian NDV isolates, 2 isolates had the virulent motif $^{112}$R-R-Q-K-R-F$^{117}$ at the cleavage site of the fusion protein while 13 isolates contained the lentogenic motif $^{112}$G-G/R-Q-G-R-L$^{117}$. Phylogenetic analysis based on the variable region of the F gene indicated that the two isolates exhibiting the virulent motif belonged to lineage 5 (genotype VII) subgenotype d and the remaining 13 isolates were grouped into lineage 2 (genotype II). The nucleotide sequences of lineage 5 isolates were genetically related to the Sudanese NDV isolates, suggesting potential epidemiological link of ND outbreaks between neighbouring countries. The lentogenic strains shared similarities with La Sota vaccine strain and probably originated from the vaccine strain either through direct exposure of birds to the live vaccine or to infectious La Sota-like strains circulating in rural poultry. This study provides genetic evidence on the existence of different NDV genotypes circulating in the rural poultry in Ethiopia. The virulent NDV continues to be a problem in poultry sector in Ethiopia, and their continuous circulation in rural and commercial poultry calls for improved surveillance and intensified vaccination and other control measures.
Starting Page	299
Ending Page	304
Page Count	6
File Format	PDF
ISSN	00494747
Journal	Tropical Animal Health and Production
Volume Number	46
Issue Number	2
e-ISSN	15737438
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-10-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Newcastle disease virus Rural chicken Pathotyping Phylogenetic analysis Zoology Veterinary Medicine
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Food Animals

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