NDLI: Development of a hybrid delta-endotoxin and its expression in tobacco and cotton for control of a polyphagous pest Spodoptera litura

Content Provider	Springer Nature Link
Author	Singh, P. Kumar, Mithilesh Chaturvedi, C. Yadav, Dinesh Tuli, Rakesh
Copyright Year	2004
Abstract	A hybrid δ-endotoxin protein was designed against a polyphagous lepidopteran insect pest Spodoptera litura, which is tolerant to most of the known δ-endotoxins. The hybrid δ-endotoxin was created by replacing amino acid residues 530–587 in a poorly active natural Cry1Ea protein, with a highly homologous 70 amino acid region of Cry1Ca in domain III. The truncated δ-endotoxins Cry1Ea, Cry1Ca and the hybrid protein Cry1EC accumulated in Escherichia coli to form inclusion bodies. The solubilised Cry1EC made from E. coli was 4- fold more toxic to the larvae of S. litura than Cry1Ca, the best known δ-endotoxin against Spodoptera sp. None of the two truncated toxins, solubilised from E. coli caused larval mortality. However, trypsinised Cry1Ca protoxin obtained from E. coli and solubilised from inclusion bodies caused mortality of S. litura with LC$_{50}$ 513 ng/ml semi synthetic diet. A synthetic gene coding for the hybrid$-endotoxin Cry1EC was designed for high level expression in plants, taking into consideration several features found in the highly expressed plant genes. Transgenic, single copy plants of tobacco as well as cotton were developed. The selected lines expressed Cry1EC at 0.1–0.7% of soluble leaf protein. Such plants were completely resistant to S. litura and caused 100% mortality in all stages of larval development. Hence, unlike in E. coli, the hybrid δ-endotoxin folded into a functionally active conformation in both tobacco and cotton leaves. The truncated Cry1EC expressed in tobacco leaves was about 8-fold more toxic (LC$_{50}$ 58 ng/ml diet) compared to expression in E. coli.
Starting Page	397
Ending Page	410
Page Count	14
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	13
Issue Number	5
e-ISSN	15739368
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Plant Sciences Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Expression of the soybean (Kunitz) trypsin inhibitor in transgenic tobacco: Effects on larval development of Spodoptera litura

Avidin Expressed in Transgenic Tobacco Leaves Confers Resistance to Two Noctuid Pests, Helicoverpa Armigera and Spodoptera Litura

Cotton α-Globulin Promoter: Isolation and Functional Characterization in Transgenic Cotton, Arabidopsis, and Tobacco

Anther-specific expression of ipt gene in transgenic tobacco and its effect on plant development

Adaptation of Spodoptera exigua (Lepidoptera: Noctuidae) to barley trypsin inhibitor BTI-CMe expressed in transgenic tobacco

Transgenic tobacco plants producing caffeine: a potential new strategy for insect pest control

Expression of the Bovine Growth Hormone Alters the Root Morphology in Transgenic Tobacco Plants

The Expression of a Mammalian Proteinase Inhibitor, Bovine Spleen Trypsin Inhibitor in Tobacco and its Effects on Helicoverpa armigera Larvae

Transgenic cotton resistant to herbicide bialaphos

Development of a hybrid delta-endotoxin and its expression in tobacco and cotton for control of a polyphagous pest Spodoptera litura

Similar Documents

Expression of the soybean (Kunitz) trypsin inhibitor in transgenic tobacco: Effects on larval development of Spodoptera litura

Avidin Expressed in Transgenic Tobacco Leaves Confers Resistance to Two Noctuid Pests, Helicoverpa Armigera and Spodoptera Litura

Cotton α-Globulin Promoter: Isolation and Functional Characterization in Transgenic Cotton, Arabidopsis, and Tobacco

Anther-specific expression of ipt gene in transgenic tobacco and its effect on plant development

Adaptation of Spodoptera exigua (Lepidoptera: Noctuidae) to barley trypsin inhibitor BTI-CMe expressed in transgenic tobacco

Transgenic tobacco plants producing caffeine: a potential new strategy for insect pest control

Expression of the Bovine Growth Hormone Alters the Root Morphology in Transgenic Tobacco Plants

The Expression of a Mammalian Proteinase Inhibitor, Bovine Spleen Trypsin Inhibitor in Tobacco and its Effects on Helicoverpa armigera Larvae

Transgenic cotton resistant to herbicide bialaphos

Development of a hybrid delta-endotoxin and its expression in tobacco and cotton for control of a polyphagous pest Spodoptera litura