NDLI: Generation of marker-free transgenic maize by regular two-border Agrobacterium transformation vectors

Content Provider	Springer Nature Link
Author	Huang, Shihshieh Gilbertson, Larry Adams, Tom Malloy, Kathleen Reisenbigler, Emily Birr, Darren Snyder, Mark Zhang, Qiang Luethy, Michael
Copyright Year	2004
Abstract	By introducing additional T-DNA borders into a binary plasmid used in Agrobacterium-mediated plant transformation, previous studies have demonstrated that the marker gene and the gene of interest (GOI) can be carried by independent T-strands, which sometimes integrate in unlinked loci in the plant genome. This allows the recovery of marker-free transgenic plants through genetic segregation in the next generation. In this study, we have found that by repositioning the selectable marker gene in the backbone and leaving only the GOI in the T-DNA region, a regular two-border binary plasmid was able to generate marker-free transgenic maize plants more efficiently than a conventional single binary plasmid with multiple T-DNA borders. These results also provide evidence that both the right and left borders can initiate and terminate T-strands. Such non-canonical initiation and termination of T-strands may be the basis for the elevated frequencies of cotransformation and unlinked insertions.
Starting Page	451
Ending Page	461
Page Count	11
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	13
Issue Number	5
e-ISSN	15739368
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Plant Sciences Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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