NDLI: Production of recombinant albumin by a herd of cloned transgenic cattle

Content Provider	Springer Nature Link
Author	Echelard, Yann Williams, Jennifer L. Destrempes, Margaret M. Koster, Julie A. Overton, Susan A. Pollock, Daniel P. Rapiejko, Karen T. Behboodi, Esmail Masiello, Nicholas C. Gavin, William G. Pommer, Jerry Patten, Scott M. Faber, David C. Cibelli, Jose B. Meade, Harry M.
Copyright Year	2008
Abstract	Purified plasma derived human albumin has been available as a therapeutic product since World War II. However, cost effective recombinant production of albumin has been challenging due to the amount needed and the complex folding pattern of the protein. In an effort to provide an abundant source of recombinant albumin, a herd of transgenic cows expressing high levels of rhA in their milk was generated. Expression cassettes efficiently targeting the secretion of human albumin to the lactating mammary gland were obtained and tested in transgenic mice. A high expressing transgene was transfected in primary bovine cell lines to produce karyoplasts for use in a somatic cell nuclear transfer program. Founder transgenic cows were produced from four independent cell lines. Expression levels varying from 1–2 g/l to more than 40 g/l of correctly folded albumin were observed. The animals expressing the highest levels of rhA exhibited shortened lactation whereas cows yielding 1–2 g/l had normal milk production. This herd of transgenic cattle is an easily scalable and well characterized source of rhA for biomedical uses.
Starting Page	361
Ending Page	376
Page Count	16
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	18
Issue Number	3
e-ISSN	15739368
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-11-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Recombinant Human albumin Mammary production Transgenic Nuclear transfer Cow Human Genetics Plant Sciences Animal Genetics and Genomics Plant Genetics & Genomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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