NDLI: Production of transgenic goats expressing human coagulation factor IX in the mammary glands after nuclear transfer using transfected fetal fibroblast cells

Content Provider	Springer Nature Link
Author	Amiri Yekta, Amir Dalman, Azam Eftekhari Yazdi, Poopak Sanati, Mohammad Hossein Shahverdi, Abdol Hossein Fakheri, Rahman Vazirinasab, Hamed Daneshzadeh, Mohammad Taghi Vojgani, Mahdi Zomorodipour, Alireza Fatemi, Nayeralsadat Vahabi, Zeinab Mirshahvaladi, Shahab Ataei, Fariba Bahraminejad, Elmira Masoudi, Najmehsadat Rezazadeh Valojerdi, Mojtaba Gourabi, Hamid
Copyright Year	2012
Abstract	There are growing numbers of recombinant proteins that have been expressed in milk. Thus one can consider the placement of any gene of interest under the control of the regulatory elements of a milk protein gene in a dairy farm animal. Among the transgene introducing techniques, only nuclear transfer (NT) allows 100 % efficiency and bypasses the mosaicism associated with counterpart techniques. In this study, in an attempt to produce a transgenic goat carrying the human coagulation factor IX (hFIX) transgene, goat fetal fibroblasts were electroporated with a linearized marker-free construct in which the transgene was juxtaposed to β-casein promoter designed to secret the recombinant protein in goat milk. Two different lines of transfected cells were used as donors for NT to enucleated oocytes. Two transgenic goats were liveborn. DNA sequencing of the corresponding transgene locus confirmed authenticity of the cloning procedure and the complementary experiments on the whey demonstrated expression of human factor IX in the milk of transgenic goats. In conclusion, our study has provided the groundwork for a prosperous and promising approach for large-scale production and therapeutic application of hFIX expressed in transgenic goats.
Starting Page	131
Ending Page	142
Page Count	12
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	22
Issue Number	1
e-ISSN	15739368
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-08-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Coagulation Factor IX Goat Transgenic Somatic cell nuclear transfer Fibroblast Molecular Medicine Plant Genetics & Genomics Animal Genetics and Genomics Plant Sciences Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Establishment and characterization of fetal fibroblast cell lines for generating human lysozyme transgenic goats by somatic cell nuclear transfer

Production of transgenic dairy goat expressing human α-lactalbumin by somatic cell nuclear transfer

Accumulation of functional recombinant human coagulation factor IX in transgenic soybean seeds

Production of Transgenic Goats, Carrying Human Coagulation Factor IX cDNA, by Nuclear Transfer of Transfected Fetal Fibroblasts

Lactation performance of transgenic goats expressing recombinant human butyryl-cholinesterase in the milk

Milk composition studies in transgenic goats expressing recombinant human butyrylcholinesterase in the mammary gland

Consumption of milk from transgenic goats expressing human lysozyme in the mammary gland results in the modulation of intestinal microflora

Production of transgenic cattle highly expressing human serum albumin in milk by phiC31 integrase-mediated gene delivery

Production of recombinant albumin by a herd of cloned transgenic cattle

Production of transgenic goats expressing human coagulation factor IX in the mammary glands after nuclear transfer using transfected fetal fibroblast cells

Similar Documents

Establishment and characterization of fetal fibroblast cell lines for generating human lysozyme transgenic goats by somatic cell nuclear transfer

Production of transgenic dairy goat expressing human α-lactalbumin by somatic cell nuclear transfer

Accumulation of functional recombinant human coagulation factor IX in transgenic soybean seeds

Production of Transgenic Goats, Carrying Human Coagulation Factor IX cDNA, by Nuclear Transfer of Transfected Fetal Fibroblasts

Lactation performance of transgenic goats expressing recombinant human butyryl-cholinesterase in the milk

Milk composition studies in transgenic goats expressing recombinant human butyrylcholinesterase in the mammary gland

Consumption of milk from transgenic goats expressing human lysozyme in the mammary gland results in the modulation of intestinal microflora

Production of transgenic cattle highly expressing human serum albumin in milk by phiC31 integrase-mediated gene delivery

Production of recombinant albumin by a herd of cloned transgenic cattle

Production of transgenic goats expressing human coagulation factor IX in the mammary glands after nuclear transfer using transfected fetal fibroblast cells