NDLI: A modified n–3 fatty acid desaturase gene from Caenorhabditis briggsae produced high proportion of DHA and DPA in transgenic mice

Content Provider	Springer Nature Link
Author	Zhu, Guiming Chen, Hongxing Wu, Xiaojie Zhou, Yanrong Lu, Jianshen Chen, Hong Deng, Jixian
Copyright Year	2008
Abstract	The functions of polyunsaturated fatty acids (PUFAs) have been widely investigated. In mammals, levels of n−3 PUFAs are relatively low compared to those of n−6 PUFAs. Either a lack of n−3 PUFAs or an excess of n−6 PUFAs could potentially cause health problems in humans. Hence, methods to increase the amount of n−3 PUFAs in diet have been intensely sought. In this study, we demonstrated that the n−3 fatty acid desaturase gene (sFat-1) synthesized from revised and optimized codons based on roundworm Caenorhabditis briggsae genomic gene for enhanced expression in mammals was successfully expressed in Chinese hamster ovary (CHO) cells and significantly elevated cellular n−3 PUFA contents. We generated sFat-1 transgenic mice by introducing mammal expression vector DNAs containing the sFat-1 gene into regular mice through the method of microinjection. Fatty acid compositions were then altered and the levels of docosahexaenoic acid (DHA, 22:6n−3) and docosapentaenoic acid (DPA, 22:5n−3) were greatly increased in these transgenic mice. Various types of tissues in the transgenic mice produced many types of n−3 PUFAs, such as alpha-linolenic acid (ALA; 18:3n−3), eicosapentaenoic acid (EPA, 20:5n−3), DPA, and DHA, for example, muscle tissues of the transgenic mice contained 12.2% DHA, 2.0% DPA, and 23.1% total n−3 PUFAs. These research results demonstrated that the synthesized sFat-1 gene with modified and optimized codons from C. briggsae possess functional activity and greater capability of producing n−3 PUFAs, especially DHA and DPA, in transgenic mice.
Starting Page	717
Ending Page	725
Page Count	9
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	17
Issue Number	4
e-ISSN	15739368
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-03-06
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	n−3 fatty acid desaturase gene Transgenic mouse Polyunsaturated fatty acids Lipids analysis Human Genetics Plant Sciences Animal Genetics and Genomics Plant Genetics & Genomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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