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Generation of Fad2 and Fad3 transgenic mice that produce n-6 and n-3 polyunsaturated fatty acids.
| Content Provider | Europe PMC |
|---|---|
| Author | Song, Lishuang Yang, Lei Wang, Jiapeng Liu, Xuefei Bai, Lige Di, Anqi Li, Guangpeng |
| Copyright Year | 2019 |
| Abstract | Linoleic acid (18 : 2, n-6) and α-linolenic acid (18 : 3, n-3) are polyunsaturated fatty acids (PUFAs), which are essential for mammalian health, development and growth. However, the majority of mammals, including humans, are incapable of synthesizing n-6 and n-3 PUFAs. Mammals must obtain n-6 and n-3 PUFAs from their diet. Fatty acid desaturase (Fad) plays a critical role in plant PUFA biosynthesis. Therefore, we generated plant-derived Fad3 single and Fad2–Fad3 double transgenic mice. Compared with wild-type mice, we found that PUFA levels were greatly increased in the single and double transgenic mice by measuring PUFA levels. Moreover, the concentration of n-6 and n-3 PUFAs in the Fad2–Fad3 double transgenic mice were greater than in the Fad3 single transgenic mice. These results demonstrate that the plant-derived Fad2 and Fad3 genes can be expressed in mammals. To clarify the mechanism for Fad2 and Fad3 genes in transgenic mice, we measured the PUFAs synthesis-related genes. Compared with wild-type mice, these Fad transgenic mice have their own n-3 and n-6 PUFAs biosynthetic pathways. Thus, we have established a simple and efficient method for in vivo synthesis of PUFAs. |
| Journal | Open Biology |
| Volume Number | 9 |
| PubMed Central reference number | PMC6833225 |
| Issue Number | 10 |
| PubMed reference number | 31640475 |
| e-ISSN | 20462441 |
| DOI | 10.1098/rsob.190140 |
| Language | English |
| Publisher | The Royal Society |
| Publisher Date | 2019-10-23 |
| Access Restriction | Open |
| Rights License | Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. © 2019 The Authors. |
| Subject Keyword | polyunsaturated fatty acids (PUFAs) n-3/6 PUFAs fatty acid desaturase (Fad) Fad2/3 transgenic mice |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology Biochemistry, Genetics and Molecular Biology Neuroscience |
