NDLI: Effect of flanking matrix attachment regions on the expression of microinjected transgenes during preimplantation development of mouse embryos

Please wait, while we are loading the content...

The restoration of fertility in male sterile tobacco demonstrates that transgene silencing can be mediated by T-DNA that has no DNA homology to the silenced transgene

Transgenic Trifolium repens with foliage accumulating the high sulphur protein, sunflower seed albumin

Expression and immune recognition of SV40 Tag in transgenic mice that develop metastatic osteosarcomas

Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response

Enhanced levels of free and protein-bound threonine in transgenic alfalfa (Medicago sativa L.) expressing a bacterial feedback-insensitive aspartate kinase gene

Role of the melanocortin-4 receptor in metabolic rate and food intake in mice

Expression of a functional mouse-human chimeric anti-CD19 antibody in the milk of transgenic mice

Corrigendum - Over-expression of the murine polymeric immunoglobulin receptor gene in the mammary gland of transgenic mice

Transgenic Research : Volume 9, Issue 1, February 2000

Transgenic Research : Volume 8

Transgenic Research : Volume 7

Transgenic Research : Volume 6

Effect of flanking matrix attachment regions on the expression of microinjected transgenes during preimplantation development of mouse embryos

Content Provider	Springer Nature Link
Author	Gutiérrez Adán, Alfonso
Copyright Year	2000
Abstract	The efficiency of transgenic animal production would increase if microinjected embryos with a successfully integrated transgene could be identified prior to transfer. It is possible to detect microinjected DNA in embryos. However, no reliable system is able to distinguish between transgenes merely present as extrachromosomal DNA and those that have been integrated into chromatin. The experiments reported here were designed to determine if the inclusion of matrix attachment regions (MARs) would enhance the efficiency of transgenic embryos identification using a selection scheme based on the expression of green fluorescent protein (GFP). Pronuclei of mouse embryos were microinjected with GFP reporter gene under the control of three different promoters and flanked or not by three different MAR elements. Transgene expression profiles were followed by direct visualization of GFP in cultured microinjected embryos. Embryos at different developmental stages were classified according to their GFP expression and groups with the same expression pattern were transferred into oviducts of pseudopregnant female mice. Fetuses were collected between days 12–15, and their genomic DNA was purified and analyzed to detect transgene integration. We did not find any statistically significant difference between the percentage of transgenic fetuses produced from GFP-positive or GFP-negative embryos transferred at 4-cell, morula, or blastocyst stage. However, when MAR elements were included in the construct, we found that GFP-positive embryos transferred at the 2-cell stage produced a significantly higher percentage of transgenic fetuses than GFP-negative embryos, but MAR sequences did not completely eliminate false positives.
Starting Page	81
Ending Page	89
Page Count	9
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	9
Issue Number	2
e-ISSN	15739368
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2000-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Plant Sciences Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The role of cell differentiation state and HMG-I/Y in the expression of transgenes flanked by matrix attachment regions

Matrix attachment regions (MARs) enhance transformation frequency and transgene expression in poplar

Analysis of Trans-Silencing Interactions Using Transcriptional Silencers of Varying Strength and Targets With and Without Flanking Nuclear Matrix Attachment Regions

Elevation of Transgene Expression Level by Flanking Matrix Attachment Regions (MAR) is Promoter Dependent: A Study of the Interactions of Six Promoters with the RB7 3′ MAR

Prediction of Transgene integration by Noninvasive Bioluminescent Screening of Microinjected Bovine Embryos

Position‐independent expression of transgenes in zebrafish

Expression and Inheritance of Nine Transgenes in Rice

A Comparison of Constitutive Promoters for Expression of Transgenes in Alfalfa (Medicago Sativa)

Mono-allelic Expression of Variegating Transgene Locus in the Mouse

Effect of flanking matrix attachment regions on the expression of microinjected transgenes during preimplantation development of mouse embryos

Similar Documents

The role of cell differentiation state and HMG-I/Y in the expression of transgenes flanked by matrix attachment regions

Matrix attachment regions (MARs) enhance transformation frequency and transgene expression in poplar

Analysis of Trans-Silencing Interactions Using Transcriptional Silencers of Varying Strength and Targets With and Without Flanking Nuclear Matrix Attachment Regions

Elevation of Transgene Expression Level by Flanking Matrix Attachment Regions (MAR) is Promoter Dependent: A Study of the Interactions of Six Promoters with the RB7 3′ MAR

Prediction of Transgene integration by Noninvasive Bioluminescent Screening of Microinjected Bovine Embryos

Position‐independent expression of transgenes in zebrafish

Expression and Inheritance of Nine Transgenes in Rice

A Comparison of Constitutive Promoters for Expression of Transgenes in Alfalfa (Medicago Sativa)

Mono-allelic Expression of Variegating Transgene Locus in the Mouse

Effect of flanking matrix attachment regions on the expression of microinjected transgenes during preimplantation development of mouse embryos