NDLI: Expression and Inheritance of Nine Transgenes in Rice

Content Provider	Springer Nature Link
Author	Wu, Liying Nandi, Somen Chen, Lifang Rodriguez, Raymond L. Huang, Ning
Copyright Year	2002
Abstract	A total of 66 transgenic rice cell lines were produced by simultaneously transforming rice callus with nine different plasmids/genes. PCR analysis indicated that the co-transformation frequency of each gene was about 70%. All the cell lines carried at least three genes and 11 cell lines carried all nine genes. Thirty-two fertile transgenic plants (R$_{0}$) were generated from the transgenic cell lines and seeds of 32 transgenic R$_{1}$ lines and 5 R$_{2}$ lines were harvested and analyzed for gene inheritance and protein expression. Progeny segregation analysis indicated that the multiple transgenes were integrated into the same locus of the rice genome, resulting in a 3:1 segregation ratio of the transgenes. Expression analysis of all nine transgenes revealed that the transgenes were expressed in all generations (R$_{0}$, R$_{1}$, and R$_{2}$) and about half of the transgenes from each line were expressed. The expression of one transgene appears to have no effect on the expression of another transgene. Among the 66 cell lines, six lines (9.1%) expressed seven or eight transgenes out of the nine transformed genes. All together, our results showed that multiple genes could be delivered into rice cells simultaneously and cell lines expressing multiple genes could be generated. The results and procedures reported here should be useful in designing multi-plasmid transformation experiments such as those required for plant metabolic engineering.
Starting Page	533
Ending Page	541
Page Count	9
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	11
Issue Number	5
e-ISSN	15739368
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2002-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Plant Sciences Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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