NDLI: The recombinant chimeric antibody chHAb18 against hepatocellular carcinoma can be produced in milk of transgenic mice

Content Provider	Springer Nature Link
Author	Wei, Jingjing Yang, Xiangmin Zheng, Min Wang, Meili Dai, Yunping Chen, Zhinan Li, Ning
Copyright Year	2010
Abstract	Biologically active recombinant monoclonal antibodies (mAbs) and their derivatives are in demand as therapeutic agents against a variety of cancers. The antibodies are generally produced by mammalian cell culture, but their production in the milk of transgenic animals would help meet the increasing demand. The mouse-human chimeric antibody chHAb18 has been proven to inhibit the invasion and metastasis of human hepatocellular carcinoma (HCC) cells by recognizing the HAb18G/CD147 molecule that is highly expressed on the surface of HCC tissue. Here, we report that transgenic mice generated by co-microinjection of two cassettes encoding the heavy and light chain genes of chHAb18 could highly express functional chHAb18 in their mammary glands. The expression level range of 1.1–7.4 mg ml$^{−1}$ was independent of transgenic copy number. Immunoassays demonstrated the ability and specificity of chHAb18 to bind purified antigen (i.e., HAb18G) or HCC cells. Recombinant chHAb18 from transgenic milk exhibited affinity almost equal to chHAb18 derived from CHO cells, and was 68% of that of the parental murine antibody, HAb18. In light of successful clinical application of HAb18, the chHAb18 expressed in mammary glands of transgenic mice constitutes an important step towards high-yield and scaled-up production of this antibody.
Starting Page	321
Ending Page	330
Page Count	10
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	20
Issue Number	2
e-ISSN	15739368
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-06-12
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hepatocellular carcinoma Chimeric monoclonal antibody Expression Transgenic mice Milk Plant Genetics & Genomics Animal Genetics and Genomics Plant Sciences Molecular Medicine Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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