Expression of a functional mouse-human chimeric anti-CD19 antibody in the milk of transgenic mice.

Content Provider	Europe PMC
Author	van Kuik-Romeijn, Petra de Groot, Nanda Hooijberg, Erik de Boer, Herman A.
Abstract	Human B cell lymphomas are suitable targets for immunotherapy. Clinical trials with mouse-human chimeric B cell-specific monoclonal antibodies (mAbs) have already shown promising results. However, limitations for their use in clinical trials can be the lack of sufficient amounts and high production costs. Expression of mAbs in the mammary gland of transgenic animals provides an economically advantageous possibility for production of sufficient quantities of a promising antibody for clinical trials and beyond. In this paper, we show the feasibility of this approach, by generating transgenic mice expressing mouse-human chimeric anti-CD19 mAbs in their milk. Mouse anti-CD19 variable (V) region genes were combined with human IgG1 heavy (H) and kappa light (L) chain constant (C) region genes and fused to the bovine β-lactoglobulin (BLG) promoter in two separate expression cassettes. Co-injection resulted in five transgenic lines. In one of these lines completely assembled chimeric mAbs were secreted into the milk, at an approximate level of 0.5mg/ml. These mAbs were able to bind specifically to the CD19 surface antigen on human B cells.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7089348&blobtype=pdf
ISSN	09628819
Journal	Transgenic Research [Transgenic Res]
Volume Number	9
PubMed Central reference number	PMC7089348
Issue Number	2
PubMed reference number	10951698
e-ISSN	15739368
DOI	10.1023/a:1008987403484
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2000-04-01
Publisher Place	Dordrecht
Access Restriction	Open
Rights License	This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. © Kluwer Academic Publishers 2000
Subject Keyword	BLG promoter chimeric expression milk monoclonal antibody mouse-human transgenic mice
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science