NDLI: Advances in transgenic rat production

Content Provider	Springer Nature Link
Author	Filipiak, Wanda E. Saunders, Thomas L.
Copyright Year	2006
Abstract	Predictable and reproducible production of transgenic rats from a standardized input of egg donors and egg recipients is essential for routine rat model production. In the course of establishing a transgenic rat service, transgenic founders were produced from three transgenes in outbred Sprague-Dawley (SD) rats and four transgenes in inbred Fischer 344 (F344) rats. Key parameters that affect transgenesis efficiency were assessed, including superovulation treatments, methods to prepare pseudopregnant recipients, and microinjection technique. Five superovulation regimens were compared and treatment with 20 IU PMSG and 30 IU HCG was selected for routine use. Four methods to prepare pseudopregnant egg recipients were compared and estrus synchronization with LHRHa and mating to vasectomized males was selected as most effective. More than 80% of eggs survived microinjection when modified pronuclear microinjection needles and DNA buffers were used. The efficiencies of transgenic production in rats and C57BL/6J (B6J) mice were compared to provide a context for assessing the difficulty of transgenic rat production. Compared to B6J mice, SD rat transgenesis required fewer egg donors per founder, fewer pseudopregnant egg recipients per founder, and produced more founders per eggs microinjected. Similar numbers of injection days were required to produce founders. These results suggest that SD rat transgenesis can be more efficient than B6J mouse transgenesis with the appropriate technical refinements. Advances in transgenic rat production have the potential to increase access to rat models.
Starting Page	673
Ending Page	686
Page Count	14
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	15
Issue Number	6
e-ISSN	15739368
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-09-29
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Transgenic rats Sprague-Dawley rats Fischer 344 rats Superovulation C57BL/6 transgenic mice Transgenic efficiency Microinjection Human Genetics Plant Sciences Animal Genetics and Genomics Plant Genetics & Genomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A survey to establish performance standards for the production of transgenic mice

Production of Transgenic Rats Using Young Sprague-Dawley Females Treated with PMSG and hCG

Strain Differences in Superovulatory Response, Embryo Development and Efficiency of Transgenic Rat Production

Fertility comparison between wild type and transgenic mice by in vitro fertilization

Efficient production of transgenic chickens based on piggyBac

Increased transgene integration efficiency upon microinjection of DNA into both pronuclei of rabbit embryos

Genetic polymorphisms among C57BL/6 mouse inbred strains

Production of mouse pups from germline transmission-failed knockout chimeras

Comparison of male chimeric mice generated from microinjection of JM8.N4 embryonic stem cells into C57BL/6J and C57BL/6NTac blastocysts

Advances in transgenic rat production

Similar Documents

A survey to establish performance standards for the production of transgenic mice

Production of Transgenic Rats Using Young Sprague-Dawley Females Treated with PMSG and hCG

Strain Differences in Superovulatory Response, Embryo Development and Efficiency of Transgenic Rat Production

Fertility comparison between wild type and transgenic mice by in vitro fertilization

Efficient production of transgenic chickens based on piggyBac

Increased transgene integration efficiency upon microinjection of DNA into both pronuclei of rabbit embryos

Genetic polymorphisms among C57BL/6 mouse inbred strains

Production of mouse pups from germline transmission-failed knockout chimeras

Comparison of male chimeric mice generated from microinjection of JM8.N4 embryonic stem cells into C57BL/6J and C57BL/6NTac blastocysts

Advances in transgenic rat production