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| Content Provider | Springer Nature Link |
|---|---|
| Author | Fielder, Thomas J. Yi, Charles S. Masumi, Juliet Waymire, Katrina G. Chen, Hsiao Wen Wang, Shuling Shi, Kai Xuan Wallace, Douglas C. MacGregor, Grant R. |
| Copyright Year | 2012 |
| Abstract | To identify ways to improve the efficiency of generating chimeric mice via microinjection of blastocysts with ES cells, we compared production and performance of ES-cell derived chimeric mice using blastocysts from two closely related and commonly used sub-strains of C57BL/6. Chimeras were produced by injection of the same JM8.N4 (C57BL/6NTac) derived ES cell line into blastocysts of mixed sex from either C57BL/6J (B6J) or C57BL/6NTac (B6NTac) mice. Similar efficiency of production and sex-conversion of chimeric animals was observed with each strain of blastocyst. However, B6J chimeric males had fewer developmental abnormalities involving urogenital and reproductive tissues (1/12, 8 %) compared with B6NTac chimeric males (7/9, 78 %). The low sample size did not permit determination of statistical significance for many parameters. However, in each category analyzed the B6J-derived chimeric males performed as well, or better, than their B6NTac counterparts. Twelve of 14 (86 %) B6J male chimeras were fertile compared with 6 of 11 (55 %) B6NTac male chimeras. Ten of 12 (83 %) B6J chimeric males sired more than 1 litter compared with only 3 of 6 (50 %) B6NTac chimeras. B6J male chimeras produced more litters per productive mating (3.42 ± 1.73, n = 12) compared to B6NTac chimeras (2.17 ± 1.33, n = 6). Finally, a greater ratio of germline transmitting chimeric males was obtained using B6J blastocysts (9/14; 64 %) compared with chimeras produced using B6NTac blastocysts (4/11; 36 %). Use of B6J host blastocysts for microinjection of ES cells may offer improvements over blastocysts from B6NTac and possibly other sub-strains of C57BL/6 mice. |
| Starting Page | 1149 |
| Ending Page | 1158 |
| Page Count | 10 |
| File Format | |
| ISSN | 09628819 |
| Journal | Transgenic Research |
| Volume Number | 21 |
| Issue Number | 6 |
| e-ISSN | 15739368 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2012-03-16 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | ES cell chimeric mice C57BL/6J JM8.N4 ES cells Animal Genetics and Genomics Molecular Medicine Plant Sciences Human Genetics Plant Genetics & Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science |
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