NDLI: Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds

Content Provider	Springer Nature Link
Author	Semenyuk, E. G. Schmidt, M. A. Beachy, R. N. Moravec, T. Woodford Thomas, T.
Copyright Year	2010
Abstract	Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFP$_{KDEL}$ reporter protein upon addition of the chemical ligand, methoxyfenozide. The chemical gene switch activated gene expression under in vitro conditions in somatic cotyledonary embryos and zygotic seed embryos cultured from transgenic soybean plants, as well as in seeds in planta under greenhouse conditions. The efficiency of induction of GFP expression under different growth conditions was strongly influenced by the developmental stage of the seed and availability of the inducer. The formation of ER-derived GFP-containing protein bodies in seed storage parenchyma cells was correlated with the level of induced expression.
Starting Page	987
Ending Page	999
Page Count	13
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	19
Issue Number	6
e-ISSN	15739368
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-02-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Inducible expression Soybean Seed-specific genes Methoxyfenozide Human Genetics Plant Sciences Animal Genetics and Genomics Plant Genetics & Genomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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