NDLI: Chemokine CXCL14/BRAK transgenic mice suppress growth of carcinoma cell xenografts

Content Provider	Springer Nature Link
Author	Izukuri, Kazuhito Suzuki, Kenji Yajima, buyuki Ozawa, Shigeyuki Ito, Shin Kubota, Eiro Hata, Ryu Ichiro
Copyright Year	2010
Abstract	We reported previously that the forced expression of the chemokine BRAK, also called CXCL14 in head and neck squamous cell carcinoma (HNSCC) cells decreased the rate of tumor formation and size of tumor xenografts compared with mock-vector treated cells in athymic nude mice or in severe combined immunodeficiency mice. This suppression occurred even though the growth rates of these cells were the same under in vitro culture conditions, suggesting that a high expression level of the gene in tumor cells is important for the suppression of tumor establishment in vivo. The aim of this study was to determine whether CXCL14/BRAK transgenic mice show resistance to tumor cell xenografts or not. CXCL14/BRAK cDNA was introduced into male C57BL/6 J pronuclei, and 10 founder transgenic mice (Tg) were obtained. Two lines of mice expressed over 10 times higher CXCL14/BRAK protein levels (14 and 11 ng/ml plasma, respectively) than normal blood level (0.9 ng/ml plasma), without apparent abnormality. The sizes of Lewis lung carcinoma and B16 melanoma cell xenografts in Tg mice were significantly smaller than those in control wild-type mice, indicating that CXCL14/BRAK, first found as a suppressor of tumor progression of HNSCC, also suppresses the progression of a carcinoma of other tissue origin. Immunohistochemical studies showed that invasion of blood vessels into tumors was suppressed in tumor xenografts of CXCL14/BRAK Tg mice. These results indicate that CXCL14/BRAK suppressed tumor cell xenografts by functioning paracrine or endocrine fashion and that CXCL14/BRAK is a very promising molecular target for tumor suppression without side effects.
Starting Page	1109
Ending Page	1117
Page Count	9
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	19
Issue Number	6
e-ISSN	15739368
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-03-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chemokine CXCL14/BRAK Tumor suppression Lewis lung carcinoma cells Transgenic mouse Tumor therapy Human Genetics Plant Sciences Animal Genetics and Genomics Plant Genetics & Genomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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Erratum to: Chemokine CXCL14/BRAK transgenic mice suppress growth of carcinoma cell transplants

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