NDLI: The human pH aldolase A promoter directs widespread but muscle-predominant expression in transgenic mice

Content Provider	Springer Nature Link
Author	Moch, Clara Spitz, Francois Porteu, Arlette Kahn, Axel Daegelen, Dominique
Copyright Year	1998
Abstract	In order to identify regulatory elements that direct widespread in vivo expression of a linked gene, we have examined one of the human aldolase A alternative promoters, the ubiquitous pH promoter, which is active in most foetal and adult tissues. We have used the pH promoter region to drive expression of an heterologous CAT reporter gene in transgenic mice. We show that a short 820 bp pH promoter fragment is able to confer a ubiquitous and reproducible activity pattern on the CAT reporter gene in most of the transgenic lines analysed, with a particularly high level of expression in adult skeletal muscle. Activity of this transgene was detected from early embryonic stages. Therefore, this pH promoter region appears to be a powerful tool to direct ubiquitous and early expression of a transgene in vivo. Deletion analysis revealed that: (i) the region between −651 and −369 bp relative to the pH promoter transcription start site includes DNA elements capable of overriding effects of the surrounding chromatin at the integration site, (ii) the region between −285 and −211 bp is involved in pH promoter tissue-specific expression pattern in skeletal muscle and/or nervous tissues, (iii) the region located between −211 and −108 bp is necessary for its ubiquitous and muscle-pre dominant activity and (iv) the most proximal region downstream from −108 bp is still sufficient to confer an activity in brain and lung
Starting Page	113
Ending Page	121
Page Count	9
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	7
Issue Number	2
e-ISSN	15739368
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1998-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Plant Sciences Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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