NDLI: An ES-like cell line from the marine fish Sparus aurata: Characterization and chimaera production

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An ES-like cell line from the marine fish Sparus aurata: Characterization and chimaera production

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Transgenic Research : Volume 11, Issue 2, April 2002

Transgenic Research : Volume 11, Issue 1, February 2002

Transgenic Research : Volume 10

Transgenic Research : Volume 9

Transgenic Research : Volume 8

Transgenic Research : Volume 7

Transgenic Research : Volume 6

An ES-like cell line from the marine fish Sparus aurata: Characterization and chimaera production

Content Provider	Springer Nature Link
Author	Béjar, Julia Hong, Yunhan Alvarez, M. Carmen
Copyright Year	2002
Abstract	Embryonic stem (ES) cells provide a unique tool for cell-mediated gene transfer and targeted gene mutations due to the possibility of in vitro selection of desired genotypes. When selected cells contribute to the germ line in chimaeric embryos, transgenic animals may be generated with modified genetic traits. Though the ES cell approach has up to now been limited to mice, there is an increasing interest to develop this technology in both model and commercial fish species, with so far promising results in the medaka and zebrafish. In this study, we present evidence regarding a long-term stable cell line (SaBE-1c), derived from embryonic cells of the aquaculture marine fish Sparus aurata which has been characterized for (i) cell proliferation, (ii) chromosome complement, (iii) molecular markers, and (iv) in vitro tests of pluripotency by alkaline phosphatase (AP) staining, telomerase activity, and induced cell differentiation. These cells have proved their pluripotent capacities by in vitro tests. Furthermore, we have demonstrated their ability to produce chimaeras and to contribute to the formation of tissues from all three embryonic germ layers. These features suggest that SaBE-1c cells have the potential for multiple applications for the ES technology in fish, with the added value of originating from an economically important species.
Starting Page	279
Ending Page	289
Page Count	11
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	11
Issue Number	3
e-ISSN	15739368
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2002-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Plant Sciences Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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