NDLI: Down-regulation of Arabidopsis DND1 orthologs in potato and tomato leads to broad-spectrum resistance to late blight and powdery mildew

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Down-regulation of Arabidopsis DND1 orthologs in potato and tomato leads to broad-spectrum resistance to late blight and powdery mildew

Content Provider	Springer Nature Link
Author	Sun, Kaile Wolters, Anne Marie A. Loonen, Annelies E. H. M. Huibers, Robin P. Vlugt, René Goverse, Aska Jacobsen, Evert Visser, Richard G. F. Bai, Yuling
Copyright Year	2015
Abstract	Multiple susceptibility genes (S), identified in Arabidopsis, have been shown to be functionally conserved in crop plants. Mutations in these S genes result in resistance to different pathogens, opening a new way to achieve plant disease resistance. The aim of this study was to investigate the role of Defense No Death 1 (DND1) in susceptibility of tomato and potato to late blight (Phytophthora infestans). In Arabidopsis, the dnd1 mutant has broad-spectrum resistance against several fungal, bacterial, and viral pathogens. However this mutation is also associated with a dwarfed phenotype. Using an RNAi approach, we silenced AtDND1 orthologs in potato and tomato. Our results showed that silencing of the DND1 ortholog in both crops resulted in resistance to the pathogenic oomycete P. infestans and to two powdery mildew species, Oidium neolycopersici and Golovinomyces orontii. The resistance to P. infestans in potato was effective to four different isolates although the level of resistance (complete or partial) was dependent on the aggressiveness of the isolate. In tomato, DND1-silenced plants showed a severe dwarf phenotype and autonecrosis, whereas DND1-silenced potato plants were not dwarfed and showed a less pronounced autonecrosis. Our results indicate that S gene function of DND1 is conserved in tomato and potato. We discuss the possibilities of using RNAi silencing or loss-of-function mutations of DND1 orthologs, as well as additional S gene orthologs from Arabidopsis, to breed for resistance to pathogens in crop plants.
Starting Page	123
Ending Page	138
Page Count	16
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	25
Issue Number	2
e-ISSN	15739368
Language	English
Publisher	Springer International Publishing
Publisher Date	2015-11-17
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Late blight Powdery mildew Resistance RNAi Susceptibility (S) gene DND1 Molecular Medicine Plant Genetics & Genomics Animal Genetics and Genomics Plant Sciences Transgenics
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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Down-regulation of Arabidopsis DND1 orthologs in potato and tomato leads to broad-spectrum resistance to late blight and powdery mildew

Down-regulation of Arabidopsis DND1 orthologs in potato and tomato leads to broad-spectrum resistance to late blight and powdery mildew

Silencing of six susceptibility genes results in potato late blight resistance

Sgt1, but not Rar1, is essential for the RB-mediated broad-spectrum resistance to potato late blight

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A new resistance gene to powdery mildew identified in Solanum neorossii has been localized on the short arm of potato chromosome 6

Fine mapping of two major QTLs conferring resistance to powdery mildew in tomato

Down-regulation of Arabidopsis DND1 orthologs in potato and tomato leads to broad-spectrum resistance to late blight and powdery mildew

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Down-regulation of Arabidopsis DND1 orthologs in potato and tomato leads to broad-spectrum resistance to late blight and powdery mildew

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Down-regulation of Arabidopsis DND1 orthologs in potato and tomato leads to broad-spectrum resistance to late blight and powdery mildew