NDLI: Overexpression of polygalacturonase-inhibiting protein 2 (PGIP2) of Chinese cabbage (Brassica rapa ssp. pekinensis) increased resistance to the bacterial pathogen Pectobacterium carotovorum ssp. carotovorum

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Overexpression of polygalacturonase-inhibiting protein 2 (PGIP2) of Chinese cabbage (Brassica rapa ssp. pekinensis) increased resistance to the bacterial pathogen Pectobacterium carotovorum ssp. carotovorum

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Overexpression of polygalacturonase-inhibiting protein 2 (PGIP2) of Chinese cabbage (Brassica rapa ssp. pekinensis) increased resistance to the bacterial pathogen Pectobacterium carotovorum ssp. carotovorum

Content Provider	Springer Nature Link
Author	Hwang, Byung Ho Bae, Hanhong Lim, Hyoun Sub Kim, Kun Bo Kim, Shin Je Im, Myoung Ho Park, Beom Suk Kim, Do Sun Kim, Jongkee
Copyright Year	2010
Abstract	Polygalacturonase-inhibiting proteins (PGIPs) are plant cell wall glycoproteins that can inhibit microbial polygalacturonase (PG) activity. In this study, we cloned five PGIP genes from Chinese cabbage (Brassica rapa ssp. pekinensis). Reverse transcription PCR expression analysis showed that the accumulation of BrPGIP transcripts differed among various tissues and in response to biotic (bacterial innoculation) and abiotic stresses (i.e., wounding, jasmonic acid, cold, NaCl, and dehydration treatment). Transcripts of BrPGIP1, BrPGIP3, and BrPGIP5 were detected in all tissues tested except the stamen, while BrPGIP2 transcripts were expressed in all tissues tested. Transcripts of BrPGIP4 were not expressed in cabbage. Innoculation with a bacterium that causes soft rot, Pectobacterium carotovorum ssp. carotovorum (Pcc), strongly induced transcripts of BrPGIP2; and expression occurred more rapidly in the resistant compared to the susceptible line. BrPGIP2 showed 50–99% similarity in amino acid sequences to extracellular PGIPs from other plants. In order to assess the role of BrPGIP2 protein in protecting plants from Pcc, we generated a number of transgenic tobacco and Chinese cabbage lines over-expressing BrPGIP2. PGIP from transgenic tobacco plants inhibited Pcc PG activity by 74%, while PGIP from wild-type tobacco plants gave only 43% Pcc PG inhibition. Transgenic Chinese cabbage plants also exhibited improved resistance to bacterial soft rot (up to 54%). PGIP from transgenic Chinese cabbage plants inhibited Pcc PG activity by 77%, while PGIP from wild-type plants showed only 8% Pcc PG inhibition. This is the first report showing the role of BrPGIPs in resistance to bacterial soft rot caused by Pcc.
Starting Page	293
Ending Page	305
Page Count	13
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	103
Issue Number	3
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-06-30
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Brassica rapa Nicotiana tabacum var. Xanthi Polygalacturonase-inhibiting proteins Soft rot resistance Pectobacterium carotovorum ssp. carotovorum Polygalacturonase Plant Pathology Plant Genetics & Genomics Plant Physiology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Horticulture

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Overexpression of polygalacturonase-inhibiting protein 2 (PGIP2) of Chinese cabbage (Brassica rapa ssp. pekinensis) increased resistance to the bacterial pathogen Pectobacterium carotovorum ssp. carotovorum

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