NDLI: Teratomas of Drosera capensis var. alba as a source of naphthoquinone: ramentaceone

Content Provider	Springer Nature Link
Author	Krolicka, Aleksandra Szpitter, Anna Stawujak, Krzysztof Baranski, Rafal Gwizdek Wisniewska, Anna Skrzypczak, Anita Kaminski, Marian Lojkowska, Ewa
Copyright Year	2010
Abstract	Plants belonging to genus Drosera (family Droseraceae) contain pharmacologically active naphthoquinones such as ramentaceone and plumbagin. Hairy root cultures obtained following Agrobacterium rhizogenes-mediated transformation have been reported to produce elevated levels of secondary compounds as well as exhibit desirable rapid biomass accumulation in comparison to untransformed plants. The aim of this study was to establish hairy root or teratoma cultures of Drosera capensis var. alba and to increase the level of ramentaceone in transformed tissue by application of abiotic and biotic elicitors. The appearance of transformed tissues—teratomas but not hairy roots was observed 18 weeks after transformation. The transformation efficiency was 10% and all teratoma cultures displayed about 3 times higher growth rate than non-transformed cultures of D. capesis. The transformation was confirmed by PCR and Southern hybridization using primers based on the A. rhizogenes rolB and rolC gene sequences. HPLC analysis of ramentaceone content indicated 60% higher level of this metabolite in teratoma tissue in comparison to non-transformed cultures. Among the elicitors tested jasmonic acid (2.5 mg l$^{−1}$) turned out to be the most effective. The productivity of ramentaceone in elicited teratoma cultures was about sevenfold higher than in liquid cultures of D. capensis var. alba and amounted to 2.264 and 0.321 mg respectively during 4 weeks of cultivation. This is the first report on the transformation of Drosera plant with A. rhizogenes.
Starting Page	285
Ending Page	292
Page Count	8
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	103
Issue Number	3
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-06-17
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Agrobacterium rhizogenes Naphthoquinone Transformation Droseracapensis var. alba Plant Pathology Plant Genetics & Genomics Plant Physiology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Horticulture

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