NDLI: Ectopic expression of SbNHX1 gene in transgenic castor (Ricinus communis L.) enhances salt stress by modulating physiological process

Content Provider	Springer Nature Link
Author	Patel, Manish Kumar Joshi, Mukul Mishra, Avinash Jha, Bhavanath
Copyright Year	2015
Abstract	The SbNHX1 gene encodes a vacuolar Na$^{+}$/H$^{+}$ antiporter that involved in the maintenance of ion homeostasis and compartmentalization of excess Na$^{+}$ or K$^{+}$ ions into the vacuole. Transgenic castor plants were developed by an improved method of Agrobacterium mediated genetic transformation using spermidine (1 mM) along with acetosyringone (200 μM), which enhanced the transformation efficiency about twofolds from 2.76 to 5.91 %. Transgenic plants were confirmed by PCR using gene (SbNHX1, hptII and gus) specific primers. The single gene integration event was confirmed by RTqPCR and Southern hybridization. Transgenic lines CL7 and CL13 showed high expression of the SbNHX1 gene compared to CL6 and CL12, therefore selected for physio-biochemical analyses, which were carried out under varying NaCl concentrations. Higher chlorophyll, RWC, K$^{+}$ content, K$^{+}$/Na$^{+}$ ratio and lower electrolytic leakage, proline, MDA, Na$^{+}$ contents compared to WT confirmed that ectopic expression of the SbNHX1 gene enhances salt tolerance of transgenic plants by modulating physiological process under stress condition. Though transgenic lines were affected under stress conditions but performed better compared to WT plants. The present study is the first report of engineering salt tolerance in castor, so far. Transgenic castor may be utilized for the cultivation in marginal salty land and thus open up the possibility of releasing arable land, which is presently under castor cultivation.
Starting Page	477
Ending Page	490
Page Count	14
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	122
Issue Number	2
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-05-13
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Abiotic stress Genetic transformation Transgenic Salicornia Salt tolerance Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Agrobacterium-mediated genetic transformation and production of semilooper resistant transgenic castor (Ricinus communis L.)

Overexpression of the Arabidopsis AtEm6 gene enhances salt tolerance in transgenic rice cell lines

Genome-wide identification of heterotrimeric G protein genes in castor (Ricinus communis L.) and expression patterns under salt stress

Overexpression of a wheat Na$^{+}$/H$^{+}$ antiporter gene (TaNHX2) enhances tolerance to salt stress in transgenic tomato plants (Solanum lycopersicum L.)

A Comparative Analysis on Morphological and Physiological Characteristics between Castor Varieties (Ricinus communis L.) under Salt Stress

Expression of the Vigna aconitifolia P5CSF129A gene in transgenic pigeonpea enhances proline accumulation and salt tolerance

Expression of the chickpea CarNAC3 gene enhances salinity and drought tolerance in transgenic poplars

Ectopic expression of a rice transcription factor, Mybleu, enhances tolerance of transgenic plants of Carrizo citrange to low oxygen stress

Ectopic overexpression of a salt stress-induced pathogenesis-related class 10 protein (PR10) gene from peanut (Arachis hypogaea L.) affords broad spectrum abiotic stress tolerance in transgenic tobacco

Ectopic expression of SbNHX1 gene in transgenic castor (Ricinus communis L.) enhances salt stress by modulating physiological process

Similar Documents

Agrobacterium-mediated genetic transformation and production of semilooper resistant transgenic castor (Ricinus communis L.)

Overexpression of the Arabidopsis AtEm6 gene enhances salt tolerance in transgenic rice cell lines

Genome-wide identification of heterotrimeric G protein genes in castor (Ricinus communis L.) and expression patterns under salt stress

Overexpression of a wheat Na$^{+}$/H$^{+}$ antiporter gene (TaNHX2) enhances tolerance to salt stress in transgenic tomato plants (Solanum lycopersicum L.)

A Comparative Analysis on Morphological and Physiological Characteristics between Castor Varieties (Ricinus communis L.) under Salt Stress

Expression of the Vigna aconitifolia P5CSF129A gene in transgenic pigeonpea enhances proline accumulation and salt tolerance

Expression of the chickpea CarNAC3 gene enhances salinity and drought tolerance in transgenic poplars

Ectopic expression of a rice transcription factor, Mybleu, enhances tolerance of transgenic plants of Carrizo citrange to low oxygen stress

Ectopic overexpression of a salt stress-induced pathogenesis-related class 10 protein (PR10) gene from peanut (Arachis hypogaea L.) affords broad spectrum abiotic stress tolerance in transgenic tobacco

Ectopic expression of SbNHX1 gene in transgenic castor (Ricinus communis L.) enhances salt stress by modulating physiological process