NDLI: Metabolomics reveals distinct methylation reaction in MeJA elicited Nigella sativa callus via UPLC

Content Provider	Springer Nature Link
Author	Farag, Mohamed A. El Sayed, Abeer M. El Banna, Ahmed Ruehmann, Susanne
Copyright Year	2015
Abstract	Cell suspension cultures are now recognized as important model for studying natural products biosynthesis and functional genomics. Nevertheless, very few studies of metabolic comparisons between cell cultures (callus) and original plants have been reported, even though the biological identity of cultured cells with the normally grown plant is of great importance. In this study, an MS-based metabolomic approach was used to compare the natural products profile of intact Nigella sativa seeds versus callus . N. sativa has been used for centuries in traditional medicine for several purposes. Its phytochemical components comprise, among others, alkaloids, saponins, flavonoids and fatty acids. Ultra performance liquid chromatography coupled to ultraviolet photodiode array detection and high resolution q-TOF mass spectrometry (UPLC–PDA–MS) was utilized to analyze the secondary product metabolome of N. sativa callus, with a total of 74 metabolites including five flavonoids, 13 hydroxycinnamates, an alkaloid, saponin and 14 fatty acids. Callus maintained the capacity to produce N. sativa phenolic subclasses, with hydroxycinnamates amounting for the major secondary metabolites in callus. Alkaloids, major constituents in Nigella genus, were detected in callus though with qualitative and quantitative differences from seed tissue. Methyl jasmonate (MeJA) elicitation effect was assessed on callus with the aim of increasing secondary metabolites production. Metabolite profiles were subjected to principal component analysis and orthogonal projection to latent structures-discriminant analysis to evaluate MeJA effect. Results revealed that MeJA led to O-methylation reaction induction yielding O-feruloylquinic acid from O-caffeoylquinic acid, in addition to a methylated disaccharide. The work extends our knowledge regarding hydroxycinnamates biosynthesis, regulation and on metabolic engineering future efforts to increase its production as potential phytoalexin in N. sativa.
Starting Page	453
Ending Page	463
Page Count	11
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	122
Issue Number	2
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-05-13
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nigella sativa Metabolomics Cell culture O-feruloylquinic acid Methyl jasmonate UPLC–MS Phytoalexins Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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