NDLI: Inorganic nitrogen uptake kinetics of sugarcane (Saccharum spp.) varieties under in vitro conditions with varying N supply

Content Provider	Springer Nature Link
Author	Hajari, Elliosha Snyman, Sandy J. Watt, M. Paula
Copyright Year	2014
Abstract	An in vitro system was established for the characterisation of inorganic nitrogen uptake by sugarcane plantlets of variety NCo376. After multiplication and rooting, plantlets (0.27–0.3 g fresh mass) were placed on N-free medium for 4 days, and then supplied with 2–20 mM N as NO$_{3}$ $^{−}$-N only, NH$_{4}$ $^{+}$-N only or NO$_{3}$ $^{−}$-N + NH$_{4}$ $^{+}$-N (as 1:1). With few exceptions, on all the tested N media, the in vitro plants always had a higher V$_{max}$ for NH$_{4}$ $^{+}$-N (28.69–66.51 μmol g$^{−1}$ h$^{−1}$) than for NO$_{3}$ $^{−}$-N uptake (10.24–30.19 μmol g$^{−1}$ h$^{−1}$) and the K$_{m}$ indicated a higher affinity for NO$_{3}$ $^{−}$-N (0.02–7.38 mM) than for NH$_{4}$ $^{+}$-N (0.06–9.15 mM). When N was applied as 4 and 20 mM to varieties N12, N19 and N36, the interaction between variety, N form and concentration resulted in differences in the V$_{max}$ and K$_{m}$. The high N-use efficient varieties (N12 and N19), as determined in previous pot and field trials, behaved similarly under all tested conditions and displayed a lower V$_{max}$ and K$_{m}$ than the low N-use efficient ones (NCo376 and N36). Based on this finding, it was suggested that the N-use efficient designation (from pot and field trials) may not be ascribed solely to N uptake. Assessment of the relative preference index (RPI) for NO$_{3}$ $^{−}$-N and NH$_{4}$ $^{+}$-N uptake revealed that, at present, the RPI has no application in sugarcane due to its preferential uptake of NH$_{4}$ $^{+}$-N.
Starting Page	361
Ending Page	371
Page Count	11
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	117
Issue Number	3
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-02-16
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ammonium Nitrate Kinetics Relative preference index Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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