NDLI: Effect of strigolactones and auxins on growth and metabolite content of Sutherlandia frutescens (L.) R. Br. microplants in vitro

Content Provider	Springer Nature Link
Author	Grobbelaar, Maria C. Makunga, kwanda P. Stander, Marietjie A. Kossmann, Jens Hills, Paul N.
Copyright Year	2014
Abstract	The influence of strigolactones as hormones in plants is not fully characterised even though they are known to affect plant architecture, both above ground and in the roots. Using an in vitro system, the effects of the synthetic auxins 1-naphthalene acetic acid and indole-3-butyric acid (NAA and IBA) and synthetic strigolactones (GR24 and Nijmegen-1) were tested on microplant development of Sutherlandia frutescens, a leguminous medicinal plant native to South Africa. Considerable phytochemical variation in wild populations has led to the proposal of using micropropagation for this species. This will assist with domestication and provide plants with a more predictable chemistry for the phytopharmaceuticals industry. Nodal explants with an axillary bud were grown on Murashige and Skoog (Plant Physiol 15:473–497, 1962) medium [0.8 % (m/v) agar (pH 5.8), 3 % (m/v) sucrose and 0.1 g/L myo-inositol] supplemented with NAA, IBA, GR24 and Nijmegen-1, either singly or in combination. The amino acid profile and secondary metabolite pool was monitored using LC–MS-profiling. Treatment with NAA promoted mass shoot production, whilst a combination of NAA and Nijmegen-1 also positively influenced the accumulation of amino acids, flavonoids (sutherlandins) and terpenoids (sutherlandiosides) that S. frutescens produces. Since these compounds represent the presumed active compounds in this species and the biomarkers used in quality control assessment of S. frutescens tissues harvested for the pharmaceutical industry, this treatment holds promise for the commercial production of Sutherlandia extracts and herbal medications.
Starting Page	401
Ending Page	409
Page Count	9
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	117
Issue Number	3
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-02-21
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Amino acids GR24 IBA NAA Nijmegen-1 Polyamines Sutherlandiosides Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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