NDLI: Molecular cloning and functional characterization of a phenylalanine ammonia-lyase from liverwort Plagiochasma appendiculatum

Content Provider	Springer Nature Link
Author	Yu, Hai Na Liu, Xin Yan Gao, Shuai Han, Xiao Juan Cheng, Ai Xia Lou, Hong Xiang
Copyright Year	2014
Abstract	Liverworts are rich in phenolic compounds, including flavonoids and the distinctive type of bisbibenzyls. The biosynthesis of both types of compounds is believed to involve the phenylpropanoid pathway. Phenylalanine ammonia-lyase (PAL) is thought to be the key enzyme in the biosynthesis of bisbibenzyls and flavonoids in liverworts. In this study, PAL (designated as PaPAL) was cloned and characterized from both the cDNA and genomic DNA of the liverwort Plagiochasma appendiculatum. The full-length cDNA sequence contains 2,202 bp and is predicted to encode a protein with 733 amino acids. Sequence alignment showed that PaPAL’s predicted amino acid sequence shares more than 70 % identity with PAL sequences reported in public databases. The recombinant protein was heterologously expressed in Escherichia coli and exhibited high PAL activity, catalyzing the conversion of l-phenylalanine to trans-cinnamic acid. However, the enzyme exhibited lower activity in catalyzing the formation of p-coumaric acid from l-tyrosine. Additionally, when the thallus of P. appendiculatum was treated with abiotic stress inducers methyl jasmonate and abscisic acid, PaPAL expression was enhanced, thereby augmenting bisbibenzyl formation. These results suggest that PaPAL plays a key role in the early steps of bisbibenzyl biosynthesis and that abiotic stress can stimulate the expression of PaPAL, resulting in the accumulation of bisbibenzyls in the plant.
Starting Page	265
Ending Page	277
Page Count	13
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	117
Issue Number	2
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-02-02
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plagiochasma appendiculatum Phenylalanine ammonia-lyase (PAL) Bisbibenzyls Phenylpropanoids Molecular cloning Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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