NDLI: Transcriptome analysis reveals the role of BpGH3.5 in root elongation of Betula platyphylla × B. pendula

Content Provider	Springer Nature Link
Author	Yang, Guang Chen, Su Jiang, Jing
Copyright Year	2015
Abstract	The GH3 family comprises a set of early auxin-responsive genes. Several previous studies have proven that GH3 family proteins play important roles in various aspects of plant growth and development. Our previous studies have found that the overexpression or suppression of BpGH3.5 in birch tree can result in shorter primary and lateral root compared to non-transgenic control, however, the molecular mechanism underlying is not clear. To further reveal the roles of BpGH3.5 in root elongation of birch (Betula platyphylla × B. pendula), we carried out transcriptome analysis on BpGH3.5-overexpressed (G1 and G5), BpGH3.5-suppressed (AG1), and non-transgenic (NT) birch. Compared with the NT line, 3,320 and 5,212 unigenes were found to be differentially expressed in the BpGH3.5 overexpressed and suppressed lines, respectively. Many differentially expressed unigenes related to cell proliferation and growth were downregulated in both the BpGH3.5-overexpressed and suppressed plants, which is consistent with the short-root phenotypes observed in these lines. We found that indole-3-acetic acid (IAA) biosynthesis and signaling pathways were affected in the BpGH3.5-overexpressed and suppressed plants. Interestingly, Free IAA levels were significantly lower in BpGH3.5-overexpressed and BpGH3.5-suppressed birch than in NT plants. This suggested that the expression levels of BpGH3.5 may play a role in maintaining the balance of free IAA in birch and the decreased IAA levels may be responsible for the short-root phenotype of the transgenic birch.
Starting Page	605
Ending Page	617
Page Count	13
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	121
Issue Number	3
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-02-06
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Birch Transcriptome BpGH3.5 IAA Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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