NDLI: Improved secondary embryo production in Quercus alba and Q. rubra by activated charcoal, silver thiosulphate and sucrose: influence of embryogenic explant used for subculture

Content Provider	Springer Nature Link
Author	Martínez, Mª Teresa Vieitez, Ana María Corredoira, Elena
Copyright Year	2015
Abstract	In somatic embryogenic systems of various oak species, including Quercus alba and Q. rubra, embryogenic lines generally proliferate through generation of nodular embryogenic structures (NS) as a form of repetitive embryogenesis, without embryo development progressing to the cotyledonary stage, or with a low proportion of embryo formation, resulting in the inability to achieve suitable material for plant recovery. In experiments we used previously initiated embryogenic lines derived from 7-year-old trees of Q. alba and Q. rubra. The aim of this work was to improve embryo proliferation and histodifferentiation of lines from these oak species to obtain well-developed embryos and plant regeneration. The effects of activated charcoal, silver thiosulphate (STS) and sucrose concentration on secondary embryo production were investigated. Overall, the best embryo proliferation medium consisted of basal Murashige and Skoog medium containing 0.4 % charcoal and 20 µM STS, and supplemented with 6 % sucrose for Q. alba and 3 % sucrose for Q. rubra. In these conditions, well-developed and singularised torpedo- and cotyledonary-shaped embryos were produced. The developmental stage of the embryogenic explant used for subculture significantly influenced embryo production and differentiation of new secondary embryos, with NS being the most effective explant despite being the smallest explant type in terms of size and fresh weight. Addition of STS in the germination medium also had a positive effect on germination response giving rise to approximately 39 % embryo conversion in Q. alba and 11 % in Q. rubra.
Starting Page	531
Ending Page	546
Page Count	16
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	121
Issue Number	3
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-01-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nodular embryogenic structures Red oak embryogenesis Somatic embryogenesis STS White oak Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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