NDLI: Cloning and characterization of FcWRKY40, A WRKY transcription factor from Fortunella crassifolia linked to oxidative stress tolerance

Content Provider	Springer Nature Link
Author	Gong, Xiao Qing Hu, Jian Bing Liu, Ji Hong
Copyright Year	2014
Abstract	The WRKY transcription factors play a key role in regulating stress responses, but the functions of most WRKYs from the non-model plants remain poorly understood. Herein, we report the isolation and functional characterization of a Group IIa WRKY gene (FcWRKY40) in Meiwa kumquat (Fortunella crassifolia). FcWRKY40 encodes a putative protein of 257 amino acids containing a coiled-coil domain and a WRKY domain. FcWRKY40 was localized in the nuclei. Transcript levels of FcWRKY40 were upregulated by salt, salicylic acid, cold and abscisic acid, but repressed by dehydration. Transgenic tobacco plants overexpressing FcWRKY40 were shown to be more tolerant to the oxidative stresses than the wild type (WT), as indicated by better morphological phenotypes of leaf or whole plant, less severe cell death, and lower MDA and H$_{2}$O$_{2}$ levels. In addition, mRNA abundances of two genes related to H$_{2}$O$_{2}$ scavenging, peroxidase (POD) and catalase (CAT), were higher in the transgenic plants than in the WT before and after oxidative stress treatment. The promoters of POD and CAT genes contained W-box elements. Both yeast one-hybrid and transient expression assay demonstrated that FcWRKY40 could interact with the two promoters. Taken together, our data suggest that FcWRKY40 functions in the oxidative stress tolerance, possibly by alleviating H$_{2}$O$_{2}$ accumulation via regulation of the antioxidant genes.
Starting Page	197
Ending Page	210
Page Count	14
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	119
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-06-11
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Abiotic stress Catalase WRKY Oxidative stress Peroxidase W-box Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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