NDLI: Douglas fir embryogenic tissue initiation

Content Provider	Springer Nature Link
Author	Pullman, Gerald S. Johnson, Shann Bucalo, Kylie
Copyright Year	2008
Abstract	Somatic embryogenesis (SE) is expected to play an important role in the future of US forests by providing increased productivity, sustainability, and uniformity. For broad scale implementation to occur, SE technology must work with a variety of genetically diverse trees. Douglas fir (Pseudotsuga menziesii (Mirb) Franco) is the dominant tree in the Pacific Northwest and has great economic and recreational value. We have developed a highly effective medium for initiation of embryogenic tissue of Douglas fir that contains ABA, biotin, brassinolide, folic acid, MES, pyruvic acid and can be used as a gelled medium or in a gelled-liquid medium overlay system. When tested with many high-value crosses over 2 years, initiation tests averaged initiation in the range of 40–57%. Additionally, a time- and labor-saving tetrazolium chloride embryo staining technique was developed to evaluate seed health and screen out seed sources likely to perform poorly in the initiation process.
Starting Page	75
Ending Page	84
Page Count	10
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	96
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-10-19
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Somatic embryogenesis Embryogenic tissue initiation Conifer Douglas fir Pseudotsuga menziesii Liquid medium overlays Plant Pathology Plant Genetics & Genomics Plant Physiology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Horticulture

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Somatic embryogenesis in loblolly pine (Pinus taeda) and Douglas fir (Pseudotsuga menziesii): improving culture initiation and growth with MES pH buffer, biotin, and folic acid

Liquid medium and liquid overlays improve embryogenic tissue initiation in conifers

Simple and efficient protocols for the initiation and proliferation of embryogenic tissue of Douglas-fir

Fraser fir somatic embryogenesis: high frequency initiation, maintenance, embryo development, germination and cryopreservation

Hygromycin promotes somatic embryogenesis in spinach

Liquid overlaying improves somatic embryogenesis in Catharanthus roseus

Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]

Embryogenic tissue initiation and somatic embryogenesis in Fraser fir (Abies fraseir [Pursh] Poir.)

Morphological and polyamine content changes in embryogenic and non-embryogenic callus of sugarcane

Douglas fir embryogenic tissue initiation

Similar Documents

Somatic embryogenesis in loblolly pine (Pinus taeda) and Douglas fir (Pseudotsuga menziesii): improving culture initiation and growth with MES pH buffer, biotin, and folic acid

Liquid medium and liquid overlays improve embryogenic tissue initiation in conifers

Simple and efficient protocols for the initiation and proliferation of embryogenic tissue of Douglas-fir

Fraser fir somatic embryogenesis: high frequency initiation, maintenance, embryo development, germination and cryopreservation

Hygromycin promotes somatic embryogenesis in spinach

Liquid overlaying improves somatic embryogenesis in Catharanthus roseus

Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]

Embryogenic tissue initiation and somatic embryogenesis in Fraser fir (Abies fraseir [Pursh] Poir.)

Morphological and polyamine content changes in embryogenic and non-embryogenic callus of sugarcane

Douglas fir embryogenic tissue initiation