NDLI: Somatic embryogenesis in loblolly pine (Pinus taeda) and Douglas fir (Pseudotsuga menziesii): improving culture initiation and growth with MES pH buffer, biotin, and folic acid

Content Provider	Springer Nature Link
Author	Pullman, Gerald S. Johnson, S. Tassel, S. Van Zhang, Y.
Copyright Year	2005
Abstract	Loblolly pine (Pinus taeda L.) somatic embryogenesis initiation was improved by supplementing the initiation medium with the pH buffer agent 2(n-morpholino)ethanesulphonic acid (MES) at 250 mg l$^{−1}$, folic acid at 0.5 mg l$^{1}$, and biotin at 0.05 mg l$^{−1}$. MES and vitamins increased the percentage of explants with extruded tissue that continued the initiation process to form embryogenic tissue. The increase in initiation was about 12%. Initiation of 12 open-pollinated families averaged 38.5%, which is 16% higher than initiation on medium without these additives. When tested with 18 control-pollinated families, initiation averaged 26.3%. Basal medium contained a combination of modified 1/2 P6 salts, activated carbon (AC) at 50 mg $^{−1}$, Cu and Zn adjusted to compensate for adsorption by AC, 1.5% maltose, 2% myo-inositol, 500 mg l$^{−1}$ casamino acids, 450 mg l$^{−1}$ glutamine, 2 mg l$^{−1}$ NAA, 0.63 mg l$^{−1}$ BAP, 0.61 mg l$^{−1}$ kinetin, 3.4 mg l$^{−1}$ silver nitrate, 10 μM 8-Br-cGMP, 0.1 μM brassinolide, and 2 g l$^{−1}$ Gelrite. Early-stage embryo growth and initiation in Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) were also improved in the presence of these additives.
Starting Page	91
Ending Page	103
Page Count	13
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	80
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	biotin conifer douglas fir folic acid initiation loblolly pine MES pH control Pinus taeda Pseudotsuga menziesii somatic embryogenesis Plant Sciences Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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Somatic embryogenesis in loblolly pine (Pinus taeda L.): improving culture initiation rates

Somatic embryogenesis in loblolly pine (Pinus taeda L.): improving culture initiation with abscisic acid and silver nitrate

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Somatic embryogenesis in loblolly pine (Pinus taeda) and Douglas fir (Pseudotsuga menziesii): improving culture initiation and growth with MES pH buffer, biotin, and folic acid

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