NDLI: Combination of site-specific recombination and a conditional selective marker gene allows for the production of marker-free tobacco plants

Content Provider	Springer Nature Link
Author	García Almodóvar, R. C. Petri, C. Padilla, I. M. G. Burgos, L.
Copyright Year	2013
Abstract	We have designed an innovative construct (pX6-DAO1) combining the chemical inducible Cre-LoxP system and the conditional selectable marker gene dao1 to obtain marker-free transgenic tobacco plants. Nicotiana tabaccum transgenic lines were regenerated on medium with 6 mM d-alanine. The DNA site-specific recombination was controlled by the inducer ß-estradiol. Regeneration on medium containing 5 μM ß-estradiol and 8 mM d-valine was not obtained. However, leaf disks from all transgenic lines regenerated in the presence of ß-estradiol, although only 9.4 % of regenerated buds developed solid marker-free shoots. Partial recombination was found in 71.7 % of buds, and no recombination was detected in only 18.9 % of buds. Nevertheless, when leaf disks from chimeric shoots were cultured in medium with 8 mM d-valine, only marker-free buds regenerated, and no partial recombinants were detected. Similarly, marker-free plants were produced from T$_{2}$ seeds, obtained from chimeric ß-estradiol-induced T$_{1}$ plants, with 100 % efficiency in selective d-valine medium.
Starting Page	205
Ending Page	215
Page Count	11
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	116
Issue Number	2
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-10-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cre-LoxP dao1 gene d-amino acid oxidase Tobacco Positive selection Negative selection Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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