NDLI: Using flow cytometry and cytological analyses to assess the genetic stability of somatic embryo-derived plantlets from embryogenic Musa acuminata Colla (AA) ssp. malaccensis cell suspension cultures

Content Provider	Springer Nature Link
Author	Escobedo GraciaMedra, Rosa M. Maldonado Borges, Josefina I. Burgos Tan, Martha J. Valadez González, Nina Ku Cauich, José R.
Copyright Year	2013
Abstract	Flow cytometry and chromosome counts were used to analyze the genetic stability of plants regenerated via Musa acuminata Colla (AA) ssp. malaccensis embryogenic cell suspension (ECS) cultures. These cultures were initiated from immature zygotic embryos (IZE) on Murashige and Skoog medium using nine different plant growth regulator (PGR) treatments. Highest percentage of embryogenic calli (EC) formation occurred on media with 4.5 μM 2,4-dichlorophenoxyacetic acid (2,4-D, 97 %), and 8.2 μM of picloram (Pi, 80 %) followed by 2.2 μM 2,4-D (75 %). Embryonic development was synchronized in liquid medium by filtration, and somatic embryo development was achieved with ECS aliquots overlaid on PGR-free medium. The EC medium composition and elapsed time of both short-term (~5 months old) and long-term (~2 years old) ECS cultures influenced plant regeneration, resulting in 65–99 % embryo germination and 50 to 100 % plant conversion. The mean 2C DNA content (1.23 ± 0.002 pg) and chromosome number (2n = 2x = 22) of M. acuminata ssp. malaccensis IZEs, seedlings and sucker plantlets were similar to the reported values. No significant differences were detected among IZEs before culturing, and none were found among the IZEs and leaves of control plants and the plants regenerated from short-term ECS lines when initiated with 2,4-D or Pi vis-à-vis the accession from which they originated. However, plants regenerated from the long-term ECS-L3 culture remained diploid, had the highest DNA content (2C = 1.283 ± 0.01 pg) and were clearly separate from the other regenerated and control plants.
Starting Page	175
Ending Page	185
Page Count	11
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	116
Issue Number	2
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-10-23
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Flow cytometry Diploid banana Immature zygotic embryos Somaclonal variation Somatic embryogenesis Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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