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| Content Provider | Springer Nature Link |
|---|---|
| Author | Zhang, Yan Min Zhang, Hong Mei Liu, Zi Hui Guo, Xiu Lin Li, Hui Cong Li, Guo Liang Jiang, Chun Zhi Zhang, Meng Chen |
| Copyright Year | 2014 |
| Abstract | Soybean is a difficult crop to manipulate through Agrobacterium tumefaciens-mediated genetic transformation. Plant–bacterium interaction plays an important role in the transformation process. Being rich with isoflavones, soybean may have an adverse effect on the A. tumefaciens-mediated genetic transformation. To investigate whether high content of endogenous isoflavones in soybean is a serious obstacle in achieving high efficient Agrobacterium-mediated transformation in soybean, a series of experiments on inhibition of isoflavone biosynthesis were conducted to improve upon soybean transformation efficiency. Results indicated that soybean isoflavones inhibited A. tumefaciens growth and respiration, the transformation efficiency [β-glucuronidase (GUS) transient expression] was negatively correlated with the phenylalanine ammonia-lyase activity and isoflavones content. The biosynthesis of soybean isoflavones was partially inhibited by sonication treatment and applying antagonists in co-culture medium and thereby decreased the adverse effects of isoflavones on Agrobacterium infection. A discernible improvement in transformation efficiency was achieved when sonication at 40 kHz for 3 min was applied along with Agro-infection and the explants were cultured on co-culture medium containing 20 μM α-aminooxyacetic acid (AOA), with the percentage of GUS transient expression as 41.4 %, being 3.6 times higher than that not sonicated and co-cultured on medium without AOA. Sonication was found not only to simply make micro-wounds for Agrobacterium to penetrate or releasing phenolic compounds for induced Agrobacterium vir gene expression; it disturbed the biosynthesis of isoflavones at the transcription level and decreased the adverse effects of isoflavones on soybean transformation, and thereby improving soybean transformation efficiency. |
| Starting Page | 183 |
| Ending Page | 193 |
| Page Count | 11 |
| File Format | |
| ISSN | 01676857 |
| Journal | Plant Cell, Tissue and Organ Culture (PCTOC) |
| Volume Number | 121 |
| Issue Number | 1 |
| e-ISSN | 15735044 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2014-12-17 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Soybean Isoflavones Inhibition Transformation Agrobacterium tumefaciens Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Horticulture |
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