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| Content Provider | Springer Nature Link |
|---|---|
| Author | Slater, Susan M. H. Keller, W. A. Scoles, G. |
| Copyright Year | 2011 |
| Abstract | An Agrobacterium tumefaciens—mediated transformation system was developed for Eruca sativa (eruca). Hypocotyl explants were co-cultivated with bacterial cells carrying a plasmid harboring a uidA:nptII fusion gene along a phosphinothricin acetyl transferase (PAT) gene cassette, for a period of 2 days. These were grown on a high cytokinin/auxin medium containing 5.0 mg l$^{−1}$ 6-benzyladenine (BA), 1.0 mg l$^{−1}$ indole-3-acetic acid (IAA), and 0.1 mg l$^{−1}$ α-naphthaleneacetic acid (NAA). Explants were then transferred to a lower cytokinin/auxin medium containing 2.0 mg l$^{−1}$ BA and 0.1 mg l$^{−1}$ NAA along with 5.0 mg l$^{−1}$ silver nitrate and 300 mg l$^{−1}$ Timentin®. Upon transfer to a selection medium containing either 20 mg l$^{−1}$ kanamycin or 2 mg l$^{−1}$ L-phosphinothricin (L-ppt), shoot regenerants were observed. Expression of the transgenes in putative transformants was confirmed using a histochemical GUS assay. Presence of the PAT transgene in GUS-positive T0 plants was confirmed by Southern blot analysis. Moreover, spot tests of T1 seedlings were conducted using the L-ppt herbicide. A transformation frequency of 1.1% was obtained with more than 60% of transgenic lines containing single copies of the transgenes. |
| Starting Page | 253 |
| Ending Page | 260 |
| Page Count | 8 |
| File Format | |
| ISSN | 01676857 |
| Journal | Plant Cell, Tissue and Organ Culture (PCTOC) |
| Volume Number | 106 |
| Issue Number | 2 |
| e-ISSN | 15735044 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2011-01-05 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Agrobacterium tumefaciens Brassicaceae Eruca sativa Plant Pathology Plant Physiology Plant Sciences Plant Genetics & Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Horticulture |
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