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  1. Plant Cell, Tissue and Organ Culture (PCTOC)
  2. Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 106
  3. Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 106, Issue 2, August 2011
  4. Agrobacterium-mediated transformation of Eruca sativa
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Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 129
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 128
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 127
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 126
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 125
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 124
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 123
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 122
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 121
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 120
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 119
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 118
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 117
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 116
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 115
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 114
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 113
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 112
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 111
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 110
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 109
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 108
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 107
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 106
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 106, Issue 3, September 2011
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 106, Issue 2, August 2011
The role of abscisic acid in plant tissue culture: a review of recent progress
A DREB gene from the xero-halophyte Atriplex halimus is induced by osmotic but not ionic stress and shows distinct differences from glycophytic homologues
Biolistic transformation of cotton (Gossypium hirsutum L.) with the phyA gene from Aspergillus ficuum
In vitro propagation of four saponin producing Maesa species
Shortening the breeding cycle of papaya (Carica papaya L.) by culturing embryos treated with ethrel
The myb transcription factor MdMYB6 suppresses anthocyanin biosynthesis in transgenic Arabidopsis
Copper-induced oxidative stress in Alternanthera philoxeroides callus
Agrobacterium-mediated transformation of Eruca sativa
A two-stage pretreatment of seedlings improves adventitious shoot regeneration in sugar beet (Beta vulgaris L.)
Cloning and characterization of a differentially expressed cDNA encoding myo-inositol-1-phosphate synthase involved in response to abiotic stress in Jatropha curcas
Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum
Influence of 5′-azacitidine on promoting recovery of cell competence for shoot organogenesis in Arabidopsis
Organogenesis and encapsulation of in vitro-derived propagules of Carrizo citrange [Citrus sinensis (L.) Osb. × Poncirius trifoliata (L.) Raf]
Characterization and pharmacological properties of in vitro propagated clones of Echinacea tennesseensis (Beadle) Small
Lauric acid improves the growth of zygotic coconut (Cocos nucifera L.) embryos in vitro
Establishment of adventitious root culture of Stevia rebaudiana Bertoni in a roller bottle system
An efficient regeneration system via somatic embryogenesis in olive
Influence of endophytic fungal elicitation on production of inophyllum in suspension cultures of Calophyllum inophyllum L.
The effect of the orientation of stem segments of grapevine (Vitis vinifera) cv. Chardonnay on callus development in vitro
A high throughput Brassica napus microspore culture system: influence of percoll gradient separation and bud selection on embryogenesis
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 106, Issue 1, July 2011
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 105
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 104
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 103
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 102
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 101
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 100
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 99
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 98
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 97
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 96
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 95
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 94
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 93
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 92
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 91
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 90
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 89
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 88
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 87
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 86
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 85
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 84
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 83
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 82
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 81
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 80
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 79
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 78
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 77
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 76
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 75
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 74
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 73
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 72
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 71
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 70
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 69
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 68
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 67
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 66
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 65
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 64
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 63
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 62
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 61
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 60
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 59
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 58
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 57
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 56
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 55
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 54
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 53
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 52
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 51
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 50
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 49
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 48
Plant Cell, Tissue and Organ Culture (PCTOC) : Volume 47

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Agrobacterium-mediated transformation of Eruca sativa

Content Provider Springer Nature Link
Author Slater, Susan M. H. Keller, W. A. Scoles, G.
Copyright Year 2011
Abstract An Agrobacterium tumefaciens—mediated transformation system was developed for Eruca sativa (eruca). Hypocotyl explants were co-cultivated with bacterial cells carrying a plasmid harboring a uidA:nptII fusion gene along a phosphinothricin acetyl transferase (PAT) gene cassette, for a period of 2 days. These were grown on a high cytokinin/auxin medium containing 5.0 mg l$^{−1}$ 6-benzyladenine (BA), 1.0 mg l$^{−1}$ indole-3-acetic acid (IAA), and 0.1 mg l$^{−1}$ α-naphthaleneacetic acid (NAA). Explants were then transferred to a lower cytokinin/auxin medium containing 2.0 mg l$^{−1}$ BA and 0.1 mg l$^{−1}$ NAA along with 5.0 mg l$^{−1}$ silver nitrate and 300 mg l$^{−1}$ Timentin®. Upon transfer to a selection medium containing either 20 mg l$^{−1}$ kanamycin or 2 mg l$^{−1}$ L-phosphinothricin (L-ppt), shoot regenerants were observed. Expression of the transgenes in putative transformants was confirmed using a histochemical GUS assay. Presence of the PAT transgene in GUS-positive T0 plants was confirmed by Southern blot analysis. Moreover, spot tests of T1 seedlings were conducted using the L-ppt herbicide. A transformation frequency of 1.1% was obtained with more than 60% of transgenic lines containing single copies of the transgenes.
Starting Page 253
Ending Page 260
Page Count 8
File Format PDF
ISSN 01676857
Journal Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number 106
Issue Number 2
e-ISSN 15735044
Language English
Publisher Springer Netherlands
Publisher Date 2011-01-05
Publisher Place Dordrecht
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Agrobacterium tumefaciens Brassicaceae Eruca sativa Plant Pathology Plant Physiology Plant Sciences Plant Genetics & Genomics
Content Type Text
Resource Type Article
Subject Horticulture
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