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| Content Provider | Springer Nature Link |
|---|---|
| Author | Wang, Cui Ting Wei, Zhi Ming |
| Copyright Year | 2004 |
| Abstract | A short-term regeneration system from leaf-base-derived callus of wheat (Triticum aestivum L.) was developed. Embryogenic callus formation and shoot regeneration were achieved from the first basal segments of 3–4-day-old seedlings. Callus formation frequency as well as plantlet regeneration frequency was dependent on the composition of basal medium and the concentration of 2,4-dichlorophenoxyacetic acid (2,4-D). MS medium with 2,4-D 4.5–9.0 μmol l$^{−1}$ was optimal for the culture of wheat leaf base. Effects of different combinations of plant growth regulators, which were added in either callus induction medium or shoot regeneration medium, were tested. Adding of BAP in callus induction medium shortened the time of shoot emergence but could not improve the producing of embryogenic calli and green plantlets. Optimal ratio of 2,4-D, BAP and NAA gave similar regeneration frequency to control. Existence of cytokinins in regeneration medium had no effect on increasing the regeneration frequency. The regenerants could grow to normal, fertile plants after they were transferred into soil. |
| Starting Page | 149 |
| Ending Page | 156 |
| Page Count | 8 |
| File Format | |
| ISSN | 01676857 |
| Journal | Plant Cell, Tissue and Organ Culture (PCTOC) |
| Volume Number | 77 |
| Issue Number | 2 |
| e-ISSN | 15735044 |
| Language | English |
| Publisher | Kluwer Academic Publishers |
| Publisher Date | 2004-01-01 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Plant Sciences Plant Physiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Horticulture |
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