NDLI: Obtaining autotetraploids in vitro at a high frequency in Citrus sinensis

Content Provider	Springer Nature Link
Author	Zhang, June Zhang, Min Deng, Xiuxin
Copyright Year	2007
Abstract	Tetraploid plants are essential for interploid hybridization to create triploid seedless citrus. Here we report a simple and efficient in vitro method for generating autotetraploids for sweet orange (Citrus sinensis). Cell-division activity in ‘Anliucheng’ sweet orange callus was analyzed using flow cytometry to determine the peak frequency of cell division at which time callus in a liquid media and solid media was treated with 1000 mg l$^{−1}$ colchicine. The percentage of the DNA-content-varied cells in the callus increased markedly from 11.0% to 44.4% and to 59.0% for liquid and solid media respectively. A total of 20 tetraploid plantlets were recovered via embryogenesis from 47 plantlets regenerated from the treated callus. All the autotetraploids were derived from different embryoids. Autotetraploids will be useful parents for interploid hybridization to generate commercially valuable seedless triploid citrus cultivars.
Starting Page	211
Ending Page	216
Page Count	6
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	89
Issue Number	2-3
e-ISSN	15735044
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2007-06-13
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Autotetraploid Callus Citrus sinensis Colchicine Flow cytometry Plant Physiology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Horticulture

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